TP53 Gene Alterations and P53 Protein Accumulation in Human Esophageal Cancer in Khartoum State, Sudan.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 11
Abstract
TP53 gene alterations were screened in 50 formalin fixed paraffin embedded (FFPE) human esophageal cancer (EC) specimens comprising 43 squamous cell carcinoma (SCC) and 7 adenocarcinoma (AC) using immunohistochemistry (IHC) and conventional PCR followed by DNA sequencing to detect p53 accumulation and mutation, respectively, in Khartoum state, Sudan. TP53 gene mutations were detected in 20 (40%) of the 50 EC cases investigated. Five out of 13 (38.5%) of SCC cases that stained positive for p53 protein, showed TP53 mutations, only one (14.3%) of seven AC samples was positive for p53 protein without associated mutation and 4/7 (57.1%) showed TP53 mutations only. Immunohistochemical stain was positive in 14/50 (28%) of EC samples; 13 (92.9%) of them were SSC cases and one (7.1%) AC. Five out of forty three (11.6%) of SSC and none of AC cases showed both immunohistochemical positive stain and mutation of TP53 gene while 8/43 (18.6%) of SSC and 1/7 (14.3%) of AC samples showed immunohistochemical positive stain but no TP53 gene mutation were present. Finally 15/50 (30%) of EC, 11 (73.3%) were SSC and 4 (26.7 %) AC showed immunohistochemical negative stain and positive TP53 gene mutations. Immunohistochemical patterns of p53 were not related significantly to mutational analysis results in the cases examined. Mutation analysis is recommended to be done on the entire length of the gene using fresh tissue to complete the whole picture in future studies.
Authors and Affiliations
Rihab M. Elfaki, Mohammed S. AbdelAziz, Hisham N. Eltayb, Munsoor M. Munsoor, Ahmed A. Gameel
Keywords
Related Articles
- EP ID EP416496
- DOI 10.9790/0853-1711086267.
- Views 53
- Downloads 0
Eagle’s Syndrome - A Rare Entity But Not So Uncommon. Alleviating Pain & Sypmtomatology
Aims and objective: Elongated styloid process is a diagnosis that should be considered in the evaluation of recurrent neck, throat or facial pain and dysphagia with or without radiation of pain to the ipsilateral ear. He...
Laryngotracheal Stenosis –Etiology And ManagementLaryngotracheal Stenosis –Etiology And Management
.
Moringa Oleifera Causes Gastro protection On Acid -Alcohol Induced Ulcer
Gastroprotective effects of Moringa oleifera was studied in 15 Wistar albino rats of both sexes weighing 350 - 450 g body weight (wt.).The rats were randomly allocated into 3 groups of 5 rats per group.Method for the pre...
“Clinical Profile Of Community Acquired Pneumonia”
Background: Community acquired pneumonia (CAP) is an acute illness acquired in the community with symptoms suggestive of lower respiratory tract infection (LRTI) together with the presence of a chest radiography of intra...
Kikuchi’s Disease: A Case Report
Kikuchi’s disease, also known as Kikuchi Fujimoto Disease or histiocytic necrotising lymphadenitis is a rare idiopathic, usually self-limiting cause of lymphadenitis. It was first described in in 1972 in japan. Clinicall...