Tracing the Pig and Cattle Origin in Processed Food and Feed Products Targeting Mitochondrial 12S rRNA Gene
Journal Title: Journal of Food Quality and Hazards Control - Year 2021, Vol 8, Issue 4
Abstract
Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed based on the 12S rRNA gene sequences of pig and cattle species from GenBank. The primers were validated by using the DNA extracted from nine different chordates, including pig, cattle, chicken, bata fish, bat, toad, African parrot, rat, and human origin. Annealing temperature ranging from 46-54 °C for 30 seconds and template DNA 1:10 serial dilutions ranging from 10 to 0.00001 ng/µl were employed for primer annealing and sensitivity analysis. Samples were analyzed using optimized Polymerase Chain Reaction (PCR) conditions. Results: The most intense expected DNA bands of pig and cattle were produced at 50 °C. Under that optimized annealing temperature pig and cattle-specific primers did not anneal with the DNA of other chordates. Total extracted DNA 0.001 ng and 0.01 ng of pig and cattle respectively containing the mitochondrial DNA (mtDNA) was successfully detected. Conclusion: These findings indicate that the newly designed primer pairs can be used to detect pig and cattle derivatives in various processed food and feed products. DOI: 10.18502/jfqhc.8.4.8256
Authors and Affiliations
D. C. Roy,S. Akhter,A. K. Sarker,M. M. K. Hossain,C. Lyzu,L. C. Mohanta,D. Islam,M. A. A. Khan,
Keywords
Related Articles
- EP ID EP699547
- DOI -
- Views 60
- Downloads 1
Whole-Genome Sequencing in Food-borne Pathogenic Bacteria
This article has no abstract. DOI: 10.18502/jfqhc.8.3.7194
Antimicrobial Interventions in Poultry Processing to Improve Shelf Life and Safety of Poultry Meat: A Review with Special Attention to Salmonella spp.
Poultry meat is one of the most popularly consumed meats worldwide. With the increased consumption, the poultry industry is also facing major challenges in maintaining of safety and shelf life of the poultry meat. Microb...
Effect of Pistacia atlantica (Bane) Essential Oil on Oxidative Stability of Sunflower Oil
Background: The antioxidant activity of Bane (Pistacia atlantica) has been proved in different researches. This study evaluated the potential of Bane (Pistacia atlantica) essential oil (as a natural antioxidant) on the o...
Extraction and Characterization of Chitosan from Snail Shells (Achatina fulica)
Background: Chitosan due to biodegradable and non-toxic characteristics has versatile applications. Extraction and characterization of Chitosan from Snail Shells In January, 2023 Achatina fulica was performed. Methods: A...
Occurrence and Antibiotic Resistance of Escherichia coli in Street-Vended Kebab in Dramaga, Bogor, Indonesia
Background: Kebab is one of the popular ready-to-eat foods in Indonesia and sold as a street food. The purpose of this study was to determine occurrence and antibiotic resistance of Escherichia coli in street-vended keba...