Turbidimetric measurement of γ-globulin in cerebrospinal fluid with use of sulfosalicylic acid and Triton X-100
Journal Title: Journal of Analytical Bio-Science - Year 2009, Vol 32, Issue 5
Abstract
We describe a simple, rapid, and cost-effective method using sulfosalicylic acid and Triton X-100 for turbidimetric measurement ofγ-globulin in cerebrospinal fluid (CSF). Measurement ofγ-globulin in CSF is an indicator of immunoglobulin production in the subarachinoid space. Immunoglobulin in CSF is assessed principally by expensive IgG assay or by semiquantitative Pandy or Nonne-Apelt tests. The method described requires 0.2 mL of CSF and 1.5 mL of precipitant, and turbidity formation measured at 660 nm completed within 15 min at 37℃. Reagent cost is approximately 1% of the cost of IgG by immunonephelometry. The turbidity was linear from 3 up to 100 mg/dL. The reagent reacted 100% withγ-globulin, 9% withα- andβ-globulins, and 1% with albumin. The within- and between-day coefficient of variation forγ-globulin was better than 5.9%. Values ofγ-globulin in CSF by our method were significantly correlated with IgG concentration by immunonephelometry (γ= 0.954, P< 0.001), and the reference interval for IgG (< 5 mg/dL) could also be adapted to our method.
Authors and Affiliations
Hiroshi Ihara
Keywords
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