Use of abscisic acid, mannitol and the reduction of the concentration of the salts of the culture medium in the in vitro conservation of Ipomoea batatas
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 1
Abstract
Nodal segment from in vitro plants of Jewel clone sweet potato were culture in the following culture medias: a) Murashige and Skoog (1962) salts supplemented with abscisic acid (0.0, 1.0, 5.0 y 10.0 mg.l-1), b) Murashige and Skoog (1962) salts supplemented with mannitol (0.0, 10.0 15.0 y 20.0 g.l-1) and c) different Murashige and Skoog (1962) salts concentration (100, 75, 50 and 25%) were used. Survival during conservation was high in all experiment. The 5.0 and 10.0 mg.l-1 abscisic acid strongly inhibited the plants growth. It was reached a decrease of growth when using mannitol in the culture medium. The media with 25% and 50% MS salts concentration caused a low of plants growth. When cultured in fresh culture medium it was achieved a high recovery ratio of conserved material.
Authors and Affiliations
Angel Espinosa Reyes, Leonardo Salas Hernández, Orlando González Paneque, Juan J. Silva Pupo
Keywords
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