Use of Human Amniotic Fluid-Derived Mesenchymal Stem Cells In Treatment Of Cisplatin-Induced Renal Injury In SpragueDawley Rats
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 4
Abstract
Background And Objectives:Cisplatin is a nephrotoxic chemotherapeutic agent. So, preventive measures worth to be evaluated. Human amniotic fluid stem cells (hAFSCs) in prevention or amelioration of cisplatininduced acute kidney injury (AKI) in Sprague-Dawley rates have been tested. Methods:80 Sprague-Dawley rats (250∼300g) were used and divided into 4 major groups, 20 rats on eachgroup. Group I:Cisplatin-injected group (7mg/kg I.P). Group II: Cisplatin-injected and hAFSCs-treated group (2×106 hAFSCs ).Group III: Cisplatin-injected and DMEM culture media treated group). Group IV: Saline-injected group. One day after cisplatin administration). Each major group was further divided into 4 equal subgroups according to the timing of sacrifice; 4, 7, 11 and 30 days post-cisplatin injection. Renal function tests were done. Kidney tissue homogenate oxidative stress parameters malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were determined. Results:hAFSCs characterization was proved. Cisplatin injection resulted in a significant increase in serum creatinine and MDA and decrease in SOD, GSH and creatinine clearance. These changes were attenuated early by day 4 with the use of hAFSCs. Cisplatin injection induced tubular necrosis, atrophy, inflammatory cells infiltration and fibrosis. The use of hAFSCs was associated with significantly lowered injury score at day 4, 7, 11 and 30 with marked regenerative changes starting from day 4. Conclusion:hAFSCs have both a protective and regenerative activities largely through an antioxidant activity. This activity cut short the acuteness of cisplatin nephrotoxicity.
Authors and Affiliations
Mohamed-A Sobh, Magdymahfoz Youssef, Heba Mohamed Reda
Keywords
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- EP ID EP415142
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