Using Comet Assay for Genotoxic Evaluation of Zataria multiflora Boiss., An Iranian Endemic Plant
Journal Title: Journal of Food Quality and Hazards Control - Year 2021, Vol 8, Issue 1
Abstract
Background: Zataria multiflora Boiss. (Avishan-e Shirazi), as an Iranian endemic plant, belongs to the Lamiaceae family and may be used as a food preservative. This study aimed to detect potential genotoxic effects of Z. multiflora extract. Methods: Hydro-alcoholic extract of Z. multiflora was prepared. Human B lymphocytes were treated with 1% extract within 3 and 24 h. Sterile Phosphate Buffered Saline (PBS) and cisplatin were used as negative and positive controls, respectively. DNA damage profiles were examined using comet assay (Single Cell Gel Electrophoresis). Data were statistically analyzed by SPSS software v. 21. Results: No statistically significant (p=0.071) DNA damage was observed in B lymphocytes treated with either Z. multiflora extract or PBS after 3 and 24 h. However, there was a statistically significant difference (p=0.0001) between DNA damage in B lymphocytes that treated with cisplatin and Z. multiflora after 3 and 24 h. Conclusion: The comet assay used in the current study showed that Z. multiflora had no genotoxic effect.
Authors and Affiliations
Z. Ayubi, A. A. Jafari, S. A. Ayatollahi-Mousavi, H. Jafari, M. Panddeh
Keywords
Related Articles
- EP ID EP692798
- DOI 10.18502/jfqhc.8.1.5463
- Views 139
- Downloads 0
High Prevalence of Mycotoxigenic Fungi and Aflatoxin B1 Contamination in Corn and Wheat Grains Grown to Albania: Implications for Food Safety
Background: Today, mycotoxins are considered critical contaminants in foodstuffs produced by fungi, highlighting the importance of food safety to human health. The toxigenic fungi invasion and mycotoxin production are hi...
Evaluation of Fascioliasis, Hydatidosis, and Tuberculosis in Domestic Animals during Post-Mortem Inspection at Jijel Slaughterhouse (Algeria)
Background: Some animal infectious diseases such as tuberculosis, hydatidosis, and fascioliasis cause public health concerns and also significant economic losses. The goal of this study was to assess the rate of fascioli...
Changes in Physicochemical Properties of Wild and Cultivated Blackberry during Postharvest Cold Storage
Background: Blackberry is a seasonal harvested fruit that is also very fragile and perishes quickly. A comparative study was conducted to find out influence of cold storage on physicochemical parameters of wild and culti...
Acrylamide Content in Food Commodities Consumed in North Macedonia and Its Risk Assessment in the Population
Background: Acrylamide (AA) is an important food contaminant resulted from Maillard reaction during thermal processing of carbohydrate rich food commodities. The present paper reports the data for the AA content in some...
Announcement of “6th International Conference on Interdisciplinary Studies in Food Industry and Nutrition Sciences of Iran” in Tehran, Iran
This article has no abstract. DOI: 10.18502/jfqhc.9.1.9683