Using Green Fluorescent Protein to Correlate Temperature and Fluorescence Intensity into Bacterial Systems
Journal Title: Journal of Nuclear Physics, Material Sciences, Radiation and Applications - Year 2016, Vol 4, Issue 1
Abstract
The unique and stunning spectroscopic properties of Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria, not to mention of its remarkable structural stability, have made it one of the most widely studied and used molecular tool in medicine, biochemistry, and cell biology. Its high fluorescent quantum yield is due to its chromophore, structure responsible of emitting green visible light when excited at 395 nm. Although it is noteworthy that there is enormous available information of the wonderful luminescent properties of GFP, the fact is that there are features and properties unexplored yet, particulary about its capabilities as molecular reporter in several biological processes. In this work, we used recombinant DNA technology to express the protein in bacteria; prepared the bacterial system both in liquid and solid media, and assembled an experimental set to expose those media to a laser beam; thereby we excited the protein chromophore and used emission spectroscopy in order to observe variations in fluorescence when the bacterial system is exposed to different temperatures.
Authors and Affiliations
K. Beltran, J. M. De Jesus-Miranda, J. A. Castro, L. A. Mandujano-rosas, J. M. Paulin-Fuentes, D. Osorio-González
Keywords
Related Articles
- EP ID EP595468
- DOI 10.15415/jnp.2016.41005
- Views 231
- Downloads 0
Determination of Cu, Zn, Mn & Fe Metals in Soil Employing the EDXRF & FAAS Techniques and Comparative Study of Results
Two analytical spectroscopic techniques viz. EDXRF and FAAS were employed in analyzing the four heavy metals i.e. Cu, Zn, Mn and Fe in 24 soil samples. The heavy metals in understudy soil were found to have very low to h...
Redistribution of Nickel Ions Embedded within Indium Phosphide Via Low Energy Dual Ion Implantations.
Transition-metal doped Indium Phosphide (InP) has been studied over several decades for utilization in optoelectronics applications. Recently, interesting magnetic properties have been reported for metal clusters formed...
PADC Detected External Neutron Field by Nuclear Tracks at RFX-mod
Measured neutron signals relevant for plasma diagnostics on Reversed Field pinch eXperiment, RFX-mod, are obtained by nuclear track methodology with PADC-NtD’s. this technique provides the external neutron field values a...
Fission Dynamics: The Quest of a Temperature Dependent Nuclear Viscosity
This paper presents a journey within some open questions about the current use of a temperature dependent nuclear viscosity in models of nuclear fission and proposes an alternative experimental approach by using systems...
Radio-Optically- and Thermally Stimulated Luminescence of Zn(BO2)2:Tb3+ exposed to Ionizing Radiation
The optical absorption of zinc tetraborate at different concentrations of the terbium impurity (0, 0.5, 1, 2, 4, 8 mol%) was analyzed. The radioluminescence (RL) emission spectra was obtained after beta irradiation of a...