UV-ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DAPOXETINE HYDROCHLORIDE AND SILDENAFIL CITRATE IN TABLET DOSAGE FORM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The objective of this research was to develop and validate a simple ultraviolet (UV) spectrophotometric method for simultaneous determination of sildenafil citrate and dapoxetine hydrochloride in a pharmaceutical formulation.Methods: Two simple UV spectrophotometric methods have been developed for simultaneous determination of sildenafil citrate and dapoxetine hydrochloride. For both methods, stock solutions were prepared in methanol followed by the further required dilutions with methanol. Proposed dual-wavelength method and ratio derivative method, the wavelength of maximum absorption for sildenafil citrate and dapoxetine hydrochloride was 292 nm and 231 nm, respectively.Results: In both methods, the linearity range lies between 10 and 60 μg/ml for sildenafil citrate and 2–12 μg/mL for dapoxetine hydrochloride with their respective wavelengths. By dual-wavelength method, the percentage of sildenafil citrate and dapoxetine hydrochloride was found to be 101.3% and 100.3%, respectively.Conclusion: Result obtained in this research work clearly indicated that both these methods were found to be accurate, precise, stable, and robust as indicated by low values of percentage relative standard deviation. Thus, the present study gives an excellent method for the determination of both the drugs in combined tablet formulation.
Authors and Affiliations
Hemesh Gadiya, Monika Maheshwari, Ashok Dashora
Keywords
Related Articles
- EP ID EP607155
- DOI 10.22159/ajpcr.2019.v12i1.29937
- Views 119
- Downloads 0
EVALUATION OF APPROPRIATE USE OF ANTIFUNGAL THERAPY IN A TERTIARY CARE HOSPITAL
ABSTRACTObjective: Appropriate use of antifungal therapy has becoming a worrying issue since misuse of antifungal may contribute to the emergence andglobal increase in antifungal resistance. Use of a more standardized ap...
ASSESSMENT OF THE GENOTOXIC AND MUTAGENIC EFFECT OF AL-TAIF POMEGRANATE (PUNICA GRANATUM L) PEEL EXTRACT ALONE AND COMBINED WITH MALATHION AND ATRAZINE PESTICIDES IN LIVER OF MALE ALBINO MICE
ABSTRACTIn our previous studies, we report the antioxidant, hepatoprotective and nephroprotective potential of of Al-Taif Pomegranate (Punica granatum L)extracts against toxicity induced by Malathion (Mal) and Atrazine (...
CONTROLLED VESTIBULAR STIMULATION: PHYSIOLOGICAL INTERVENTION IN DIABETES CARE
ABSTRACTObjective: The present study was aimed to assess the role of vestibular stimulation in the management of diabetes care.Methods: We carried out the present study in 83-year-old man with 20 years history of Type 2...
IMPACT OF RAPID EYE MOVEMENT SLEEP DEPRIVATION ON HYPOTHALAMIC-PITUITARYTESTICULAR AXIS IN WISTAR ALBINO RATS
Objectives: The objectives of the study were to study the impact of rapid eye movement (REM) sleep deprivation (SD) on the hypothalamic-pituitary-testicular (HPT) axis in the Wistar albino rats.Methods: Adult male Wistar...
STUDY ON ANTIBACTERIAL EFFECTS OF SEVERAL VIETNAMESE MEDICINE PLANTS AND THEIR RELATIONSHIPS WITH POLYPHENOL CONTENTS
Objective: Our study evaluated the inhibitory effects of 13 plant materials on bacterial growth, so as to explain their traditional uses in bacterial infections. In addition, the correlation between antibacterial effects...