Validated Chromatographic method for Estimation of CoQ10 in Bulk Drug and SelfNanoemulsifying Formulation
Journal Title: International Journal of Pharmaceutical and Medicinal Reserach - Year 2016, Vol 4, Issue 1
Abstract
A simple, precise and sensitive RP-HPLC method was developed and validated for the routine analysis of CoQ10 in bulk drug and self-nanoemulsifying formulation. The analyte was quantified using column C18 which configurationally has a binary pump system, a UV-detector and an injector. The mobile phase consisted of methanol and n-hexane in the ratio of (80:20 % v/v) flowing gradiently at a flow rate of 1 ml/min. The wavelength monitored at 275 nm for estimation of drug. The previously weigh quantity of drug was transferred in mobile phase, filtered through membrane of pore size 0.45 micron and injected into column. The designed parameter was validated for specificity, linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) and robustness. The graph was linear over the concentration range of 0.010-10 µg/ml with a correlation coefficient R2 = 0.999. The LOD and LOQ value of CoQ10 was determined 0.0032 and 0.01 µg/ml. The method was successfully validated as per ICH guidelines and found to be simple, precise and accurate for the estimation of CoQ10. It has great applicability in assessing CoQ10 in self-nanoemulsifying formulation developed in the lab and other marketed formulation.
Authors and Affiliations
MD. Habban Akhter, Devendra S. Rathore
Keywords
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