VALIDATED ESTIMATION OF CAPECITABINE BY UV-SPECTROSCOPIC, RP-HPLC AND HPTLC METHOD
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 11
Abstract
This paper describes the Quantitative estimation of Capecitabine in pure powder and its pharmaceutical dosage form by UV spectroscopy (geometric correction method), RP – HPLC and HPTLC methods. In the UV method, geometrical correction procedures have been developed which reduce or eliminate the background irrelevant absorption that may be present in samples of biological origin. In this method, three wavelengths 263, 281, and 293 nm were selected for the estimation of Capacitabine in Serum. Based on the absorbance the corrected absorbance was calculated. Capecitabine was quantified by using the corrected absorbance. In the RP-HPLC method, the drug was resolved using a mobile phase [methanol: buffer (1 ml glacial acetic acid in1000 ml water) (45:55 % v/v)] on C18 column [develosil ODS-MG-5 (100 nm 4.6 mm) 5] in isocratic mode. The retention time of Capecitabine was found to be 2.17 min. In HPTLC method, the chromatograms were developed by using a mobile phase [Chloroform: glacial acetic acid: methanol (8:1:1 % v/v)] on precoated plate of silica gel 60F254 and quantified by CAMAG TLC scanner 3, at 240 nm. The Rf value of Capecitabine was 0.271. Mean Recovery is 98.8-101.14%, percentage relative standard deviation (%RSD less than 2%) and correlation coefficient (linearity range) that developed methods were accurate and precise. These methods can be employed for the routine analysis of tablets containing Capecitabine.
Authors and Affiliations
Rajitha kandimalla, D. Nagavalli
Keywords
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