Validated method for estimation of irbesartan in bulk and dosage form by high performance liquid chromatography technique
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2015, Vol 3, Issue 3
Abstract
A simple, specific, accurate and precise new high performance liquid chromatographic method was developed for the estimation of irbesartan in bulk and its developed dosage form. The mobile phase containing acetonitrile: Phosphate buffer pH 3.5 in proportion of 50:50 v/v was employed with flow rate of1.0 ml/min and eluting medium was monitored at 240 nm. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and robustness for irbesartan. A linear response was observed in the range of 5- 40μg/ml. Linear regression of absorbance on concentration gave equation y = 101.9x + 195.3 with a regression co-efficient r2=0.993. The method was validated for different parameters as per the ICH guidelines. The degradation studies were carried out by using the developed method. Thus the method was found to be useful for the determination of irbesartan in bulk as well as for dosage forms.
Authors and Affiliations
N. S. Kulkarni| NIMS institute of Pharmacy, NIMS University, Jaipur, Rajasthan, India, D. S. Rathore| NIMS institute of Pharmacy, NIMS University, Jaipur, Rajasthan, India, N. S. Ranpise| Sinhgad college of Pharamcy, Vadgaon (Bk), Pune, Maharashtra, India, S. N. Dhole| PES, Modern college of Pharmacy (For Ladies), Moshi, Pune, Maharashtra, India
Keywords
Related Articles
- EP ID EP15345
- DOI -
- Views 295
- Downloads 14
Role of Rauwolfia serpentina in stroke induced experimental dementia
Objective: To investigate the Role of Rauwolfia serpentina in stroke induced experimental dementia. Methods: Methanolic root extract of Rauwolfia serpentina(RS) at a dose of 10mg/kg,20mg/kg and 40mg/kg;p.o. was studied a...
Formulation and evaluation of Acyclovir microparticles for Ocular Delivery
The objective of the present work was to formulate and evaluate microparticles of Acyclovir and produced sustained drug delivery for ocular delivery. In this 9 batches(A1-C3) of acyclovir microparticle was prepared wit...
Development of Anti-diabetic Niosomes Formulation Containing Metformin and Gliclazide
Metformin/Gliclazide niosomes were formulated with span 60 by ether injection method. Three batches MG1-MG3 were prepared in order to study influence of drug polymer ratio on the niosomes formation and in vitro drug re...
A Review on Pharmaceutical Validation and Its Implications
The process validation is establishing documented evidence which provides high degree of assurance that a specific process consistently produces a product meeting its predetermined specifications and quality characteris...
Tissue esterases of Lamellidens corrianus (Fresh water mussel)
Polyacrylamide gel electrophoretic (PAGE) technique was used to examine the esterase pattern of Ctenidia, Hepatopancreas, Hemolymph, Intestine, Mantle. Foot of the Lamellidens corrianus. The zymograms revealed the var...