Validated method for estimation of irbesartan in bulk and dosage form by high performance liquid chromatography technique
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2015, Vol 3, Issue 3
Abstract
A simple, specific, accurate and precise new high performance liquid chromatographic method was developed for the estimation of irbesartan in bulk and its developed dosage form. The mobile phase containing acetonitrile: Phosphate buffer pH 3.5 in proportion of 50:50 v/v was employed with flow rate of1.0 ml/min and eluting medium was monitored at 240 nm. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and robustness for irbesartan. A linear response was observed in the range of 5- 40μg/ml. Linear regression of absorbance on concentration gave equation y = 101.9x + 195.3 with a regression co-efficient r2=0.993. The method was validated for different parameters as per the ICH guidelines. The degradation studies were carried out by using the developed method. Thus the method was found to be useful for the determination of irbesartan in bulk as well as for dosage forms.
Authors and Affiliations
N. S. Kulkarni| NIMS institute of Pharmacy, NIMS University, Jaipur, Rajasthan, India, D. S. Rathore| NIMS institute of Pharmacy, NIMS University, Jaipur, Rajasthan, India, N. S. Ranpise| Sinhgad college of Pharamcy, Vadgaon (Bk), Pune, Maharashtra, India, S. N. Dhole| PES, Modern college of Pharmacy (For Ladies), Moshi, Pune, Maharashtra, India
Keywords
Related Articles
- EP ID EP15345
- DOI -
- Views 341
- Downloads 14
Hepatoprotective potential of Trigonella foenum graecum in deltamethrin induced albino rats
Objective: Aim of investigation focuses attention on hepatoprotective and antioxidative effect of aqueous extract of Trigonella foenum graecum (TFG) in hepatic tissue of deltamethrin fed rats. Methods: In a 45 days tre...
Preliminary Pharmacological Screening of Ethanolic Extract of Aristoochia indica on Nephrolithiasis Rat
The effect of ethanolic extract of Aristolochia indica was studied on experimentally induced nepharolithatic and urolithuasis in rats. Oxalate urolithiasis was produced by the addition of 0.75% ethylene glycol in the d...
Combination of Loperamide and Niacin modify DENA induced altered Liver enzymes
Hepatocellular Carcinoma (HCC) is among the most lethal cancers which makes it the third most frequent cause of cancer related deaths. Diethylnitrosamine (DENA) is a potent initiator and hepatocarcinogen in rats. DENA...
Development of Anti-diabetic Niosomes Formulation Containing Metformin and Gliclazide
Metformin/Gliclazide niosomes were formulated with span 60 by ether injection method. Three batches MG1-MG3 were prepared in order to study influence of drug polymer ratio on the niosomes formation and in vitro drug re...
Direct multiple shoots proliferation of black night shade (Solanum nigrum l.) from shoot tip explants induced by thidiazuron
The present investigation was undertaken in Black Night Shade (Solanum nigrum L.) which is an important medicinal plant. Direct multiple shoots proliferation was achieved from shoot tip. The shoot tips were cultured on...