VALIDATED REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL, HYDROCHLOROTHIAZIDE AND AMLODIPINE BESYLATE IN NEWLY DESIGNED PHARMACEUTICAL DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 11
Abstract
A Simple, accurate, and precise reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Olmesartan Medoxomil, Hydrochlorothiazide and Amlodipine Besylate in marketed formulation is developed. The determination was carried out on a Zorbax SB Phenyl C18 (250 x 4.6 mm, 5 µm) column using a mobile phase of Sodium Perchlorate Buffer (pH 3) Buffer solution: 10% THF Containing Acetonitrile (60:40 v/v). The flow rate was 1 ml/min with detection at 250 nm. The retention time for Hydrochlorothiazide was 4.0 min, Amlodipine Besylate was 10.44 min, and for Olmesartan Medoxomil was 13.8 min. Olmesartan medoxomil, Hydrochlorothiazide and Amlodipine besylate showed a linear response in the concentration range of 10-70 µg/ml, 6.25 - 43.75 µg/ml and 2.5 – 17.5 µg/ml respectively. The correlation co-efficient (' r ' value) for Olmesartan medoxomil, Hydrochlorothiazide and Amlodipine besylate was 0.9997, 0.9999 and 0.9999 respectively. The results of analysis have been validated as per ICH guidelines and by recovery studies. The percentage recoveries obtained for Olmesartan medoxomil, Hydrochlorothiazide and Amlodipine besylate ranges from 99.9% to 101.1%, 99.1% to 99.9% and 99.7% to 100.7% respectively.
Authors and Affiliations
Naman Doshi, Avani Sheth, C N Patel
Keywords
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