VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF IVABRADINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2017, Vol 9, Issue 5
Abstract
A simple, rapid, precise, and accurate RP-HPLC method for the estimation of Ivabradine Hydrochloride an anti-anginal agent, both as a bulk drug and in pharmaceutical formulation was developed. The chromatographic separation was achieved on a Thermosil C18 150 × 4.5 mm, 5μm column by using a mobile phase containing a mixture of methanol and phosphate buffer pH 6.5 in the ratio of 65:35 % v/v at a flow rate of 1ml/min and at an ambient temperature. The detection was monitored at a wavelength of 265nm. A clear chromatographic peak was identified with the retention time of 4.36 min and tailing factor of 1.23. The developed method was validated according to ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method shows a good linear relationship with correlation co-efficient of more than 0.992 in the concentration range of 30µg-150µg. The method showed mean % Recovery of 100.4% and %RSD for repeatability and intermediate precision was less than 2%. The proposed method can be used successfully for the quantitative determination of Ivabradine HCL in pharmaceutical dosage forms.
Authors and Affiliations
Md. Muzaffar-ur- Rehman, G. Nagamallika
Keywords
Related Articles
- EP ID EP618310
- DOI 10.25004/IJPSDR.2017.090505
- Views 86
- Downloads 0
ANTIMICROBIAL ACTIVITY OF WHOLE PLANT OF LUFFA CYLINDRICA (LINN) AGAINST SOME COMMON PATHOGENIC MICRO-ORGANISMS
In this present investigation was to evaluate the antimicrobial activity of pet ether and chloroform extract of whole plant of Luffa cylindrica (Linn) by in-vitro method in agar plate against ten bacterial and four funga...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF EMBELIN IN EMBELIA TSJERIAM COTTAM
Embelin, a naturally occurring benzoquinone, is obtained from fruits of Embelia tsjeriam cottam, which plays a vital role in the Ayurvedic system of medicine. An approach for the stress degradation was successfully appli...
ANTIANXIETY ACTIVITY OF FRACTIONS AND ISOLATED COMPOUNDS OF VERBENA OFFICINALIS AERIAL PARTS
Verbena officinalis L. (Vervain; family-Verbenaceae) has long been used in the treatment of mental disorders, but not validated systemically for traditional claims. The ethyl acetate fraction (rich in flavonoids) of V. o...
DEVELOPMENT AND VALIDATION OF SIMPLE UV-SPECTROPHOTOMETRIC METHOD OF QUANTIZATION OF ONDANSETRON HYDROCHLORIDE IN SOLID DOSAGE FORMULATIONS USING HYDROTROPIC SOLUBILIZATION TECHNIQUE
Commonly used organic solvents for spectrophotometric analysis of water insoluble drugs are methanol, ethanol, chloroform, benzene, toluene etc. The main drawbacks of organic solvents include high cost, toxicity, and pol...
HUTCHINSON-GILFORD PROGERIA SYNDROME: A PREMATURELY AGING DISORDER
Hutchinson-Gilford Progeria Syndrome (HGPS) is an extremely rare genetic disorder characterized by premature aging, involving aberrant splicing of the LMNA gene, resulting in the production of a disease-causing mutant la...