Validation of detection method for tributyl phosphate residue in human prothrombin complex
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 6
Abstract
Objective To establish gas chromatography for the determination of tributyl phosphate(TBP) residues in human prothrombin complex and then verify it. Methods Acid modified polyethylene glycol(PEG)(20M) capillary column was used with n-hexane as solvent. The chromatographic parameters were as follows: gasification chamber temperature at 220 ℃, column temperature at 155 ℃, detector temperature at 220 ℃, column flow rate at 2.0 mL/min, carrier gas as N2, detector as FID, and collection time for 10min. The accuracy, repeatability, linearity, specificity, intermediate precision, detection limit, quantitative limit, range and durability were verified. Results The verification results showed that the method had good specificity. The linear regression correlation coefficient of standard curve was 0.999 90. The recovery rate were 98.4%, 97.5% and 95.7% when the concentration at 50%, 100%(30μg/mL) and 150%, respectively, with an average recovery of 97.2% and a relative deviation of 2.15%. When the concentration was 100%, the repeatability was 2.08%, and the relative deviation of intermediate precision was 1.63%. The detection limit was 0.255 μg/mL, and the quantitative limit was 0.511 μg/mL. After changing capillary chromatographic columns with different batch numbers but the same types and manufacturer, the applicability test of the system met the requirements, and the method had good durability. Conclusion This method can be used for the determination of TBP residues of human prothrombin complex in laboratory.
Authors and Affiliations
Mingxiang SONG, Qiming ZHANG, Zi DOU, Yunfei ZHAO, Xingye ZHU, Xiaojie ZHAO, Jipeng ZHANG, Anshan ZHAGN, Xiao LIU
Keywords
Related Articles
- EP ID EP738274
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.06.016
- Views 36
- Downloads 0
Construction of a whole business process supervision and management system: based on management information system of blood banks
Objective To achieve supervision and management of the whole business process of blood center, raise productivity and ensure blood quality by enabling blood center managers comprehensively grasp the key business operatio...
Analysis on testing ability of blood testing laboratories in domestic blood establishments across China
Objective To analyze testing ability of blood testing laboratories in domestic blood establishments, and to comprehensively understand the resource allocation, workload and unqualified blood samples. Methods All blood te...
The capacity evaluation and utilization strategy of the platelet donor database with known HLA/HPA genotype in Shaanxi
Objective To evaluate the appropriate optimal capacity and matching probability of the platelet donor database with known HLA/HPA genotype in Shaanxi aera, and provide data support for subsequent construction, maintenanc...
Material compatibility of human albumin
Objective To investigate the compatibility of human albumin and its internal packaging materials of Sinopharm Lanzhou Biopharmaceutical Co., Ltd. Methods One batch of inner packaging materials (medium borosilicate glass-...
Optimization of blood testing strategies for voluntary blood donors in military blood centers
Objective To explore the optimization of blood testing strategies for voluntary blood donors in military blood centers. Methods From January 2017 to December 2020, the testing results of five serological indicators of vo...