VALIDATION OF HPTLC METHOD FOR QUANTITATIVE ESTIMATION OF L-DOPA FROM MUCUNA PRURIENS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 4
Abstract
In the present study, high performance thin-layer chromatographic method was developed and validated for the quantification of L-Dopa from Mucuna pruriens (Black-coloured seeds). TLC aluminium plates precoated with silica gel were used as the stationary phase in a twin see through glass chamber saturated with n-butanol: glacial acetic acid :water 4:1:1 (v/v), as the mobile phase, performed at room temperature (25 ± 2°C). The Rf value of L-Dopa was found to be 0.39. Linearity was found to be in the concentration range 100–1200 ng/spot. The linear regression analysis of calibration plots showed good linear relationship between peak area and peak height (r2 = 0.996). The method was validated for precision, recovery, robustness, specificity, detection and quantification limits. The limits of detection and quantification were 0.010 and 3.14 ng/ spot, respectively. The recovery was 100.5 %. Statistical analysis of data proves that the method is reproducible for the analysis of L-Dopa.
Authors and Affiliations
Uma Sundaram , P. Gurumoorthi
Keywords
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