VARIABILITY OF CAROTENOID-SYNTHETIC STRAINS STREPTOMYCES GLOBISPORUS 1912 AFTER DEEP CULTIVATION AND STORAGE
Journal Title: Мікробіологія і біотехнологія - Year 2018, Vol 0, Issue 3
Abstract
Aim. To determine the variability of the trait of carotenoids biosynthesis in two mutant strains Streptomyces globisporus 1912 after deep cultivation and storage. Relevance. Study of the spontaneous variability of strains-producers of carotenoids after deep cultivation and long-term storage is necessary for understanding the mechanisms of intrapopulation inheritance and the possibility of their control. Methods. Visually the analysis of phenotype of streptomyces colonies, statistical analysis was performed with Windows Software Excel 2007. Results. The appearance of non-productive/non-active variants (yellow, creamy and colorless) in populations of strains S. globisporus 4Lcp-Hp7 and S. globisporus 7Crt after cultivation in corn-soy liquid medium was 10-3 and 10-2, at 1–2 orders of magnitude higher than their standard cleavage with frequent transplantation of subcultures. The variability during storage had high indexes, which were dependent on storage temperature. Non-productive variants of strains S. globisporus 4Lcp-Hp7 and S. globisporus 7Crt at storage temperature of 4 °C were about 50% and 10% of the population, respectively. There were near 10% non-active colonies in the population of strain S. globisporus 4Lcp-Hp7, and 20–30% ones in the case strain S. globisporus 7Crt at storage temperatures of 21 °C and 28 °C. After one-year storage near 52% and 66% colonies of the populations of lyophilized cultures S. globisporus 4Lcp-Hp7 and S. globisporus 7Crt supported the high level of synthesis of corresponding carotenoids. Conclusions. It has been shown, that the trait of carotenoids biosynthesis in the mutant strains S. globisporus 4Lcp-Hp7 and S. globisporus 7Crt was showing variability with the high frequency after deep cultivation (10-3 and 10-2 non-active colonies) and during long storage on slants and in lyophilized state (10–50%).
Authors and Affiliations
S. L. Golembiovska, T. V. Dvornyc, L. V. Polishchuk, B. P. Matselykh
Keywords
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- EP ID EP396749
- DOI 10.18524/2307-4663.2018.3(43).142234
- Views 84
- Downloads 0
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