Variants of Tribulus species – a scientific study through DNA RAPD – molecular characterization
Journal Title: Indian Journal of Pharmaceutical and Biological Research - Year 2014, Vol 2, Issue 4
Abstract
Gokshura a well-known drug in Ayurveda which is extensively used in many disease conditions like dysuria, asthma, diabetes, cough, oedema, cardiac disorders etc. Tribulus terrestris (Family – Zygophyllaceae) is an official source of Gokshura as per API. Five species of genus Tribulus are found throughout India with a slight morphological difference. In this study, three different species of Tribulus genus from different regions were subjected for molecular characterization by RAPD method. Analysis showed that three different samples gave clearly similar banding pattern with each of the random primers used and 80% similarity between the three samples were observed when the results were subjected to band scoring and analysis with clustering. Even through the micromorpholgical observations showed differentiating characters in mature carpels and intrastaminal glands of the selected species.
Authors and Affiliations
Bhavesh Patil| PhD scholar, Department of Dravyaguna, IPGT & RA, Gujarat Ayurved University, Jamnagar, Gujarat, India, Bhupesh Patel| Assistant professor, Department of Dravyaguna, IPGT & RA, Gujarat Ayurved University, Jamnagar, Gujarat, India, Harisha CR| Head, Pharmacognosy Laboratory, IPGT & RA, Gujarat Ayurved University, Jamnagar, Gujarat, India, Neha Parmar| PhD scholar, Department of Dravyaguna, IPGT & RA, Gujarat Ayurved University, Jamnagar, Gujarat, India, Ashwini Save| Ayurveda Consultant, Shree Sai Ayurveda Clinic, Palghar, Maharashtra, India
Keywords
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