A Green Approach For The Simultaneous Estimation Of Gatifloxacin And Flurbiprofen by a Very Sensitive Spectrofluorimetric Method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 1
Abstract
Gatifloxacin (GAT), an antibiotic and Flurbiprofen (FLU), a Non-steroidal anti-inflammatory drug are co-formulated as an ophthalmic solution for the reduction of post operative ocular inflammation & ocular infections. Quality control of the ophthalmic solution of GAT and FLU is very essential and hence this work aims at the development of an economic and ecofriendly method for simultaneous estimation of GAT and FLU in an ophthalmic solution. Spectrofluorimetric method is selected due to the known fact of its inherent sensitivity. 10mM SLS was used as the solvent and micellar solubilisation resulted in an enhanced fluorescence for GAT and FLU. The excitation wavelengths selected for GAT and FLU were 294 nm and 259 nm respectively. The emission wavelengths for GAT and FLU were 470 nm and 315 nm respectively. A linear relationship was found in the concentration range of 0.005-2.5 μg/mL and 0.005-0.35 μg/mL for GAT and FLU, respectively. The percentage recovery was 98.2-100.1% for GAT and 98.6-102% for FLU. Keywords: Flurbiprofen, Gatifloxacin, Sodium lauryl sulphate, ValidationGatifloxacin (GAT), an antibiotic and Flurbiprofen (FLU), a Non-steroidal anti-inflammatory drug are co-formulated as an ophthalmic solution for the reduction of post operative ocular inflammation & ocular infections. Quality control of the ophthalmic solution of GAT and FLU is very essential and hence this work aims at the development of an economic and ecofriendly method for simultaneous estimation of GAT and FLU in an ophthalmic solution. Spectrofluorimetric method is selected due to the known fact of its inherent sensitivity. 10mM SLS was used as the solvent and micellar solubilisation resulted in an enhanced fluorescence for GAT and FLU. The excitation wavelengths selected for GAT and FLU were 294 nm and 259 nm respectively. The emission wavelengths for GAT and FLU were 470 nm and 315 nm respectively. A linear relationship was found in the concentration range of 0.005-2.5 μg/mL and 0.005-0.35 μg/mL for GAT and FLU, respectively. The percentage recovery was 98.2-100.1% for GAT and 98.6-102% for FLU.
Authors and Affiliations
Ceema Mathew1, Nikitha Mala, Ajitha Makula,, Sathesh Babu Puvvadi
Keywords
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- EP ID EP383358
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