A Novel Improved RP-HPLC Method for the Estimation of Dothiepin Hydrochloride in Bulk and Pharmaceutical Dosage Form
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 3
Abstract
A Simple and precise reverse phase high performance liquid chromatography(RP-HPLC)method was developed and validated for the estimation of Dothiepin hydrochloride in bulk and pharmaceutical dosage form. The optimization is carried out on an Inertsil ODS 3 (250×4.6mm, 5µ particle size) long column with a mobile phase of Formic acid(0.02%): Acetonitrile in the ratio of 72:28v/v at a flow rate of 1ml/min with UV detection at 230 nm using LC solution software. The Linearity of Dothiepin hydrochloride was observed in the concentration range of 2-10µg/ml (R2=0.999). The proposed method has shown consistent recovery of Dothiepin 99.41-100.85% with the labeled amount in the pharmaceutical formulation. The proposed method was successfully applied for the quantification of active drug in tablet dosage form.
Authors and Affiliations
Vijaya lakshmi . M, M. Srinavyatha,, Lakshmi Prasanna. M, Buchi N. Nalluri
Keywords
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- EP ID EP388856
- DOI 10.9790/3008-1203012126
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