A NOVEL STABILITY INDICATING UPLC METHOD FOR THE ESTIMATION OF TEZACAFTOR AND IVACAFTOR IN TABLET DOSAGE FORM
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 11
Abstract
UPLC is a modern technique which refers to Ultra Performance Liquid Chromatography and enhances in three main areas: Speed, resolution, and sensitivity. In this present study, an accurate and precise UPLC method was developed and validated for the stability indicating assay method to estimate Tezacaftor and Ivacaftor simultaneously in both bulk and tablet dosage form. This method was developed using column HSS C18 (100 × 2.1mm 1.7m) with mobile phase 0.1% OPA Buffer: acetonitrile taken in the ratio 50:50. The flow rate was 0.3 ml/min with an 1 μl injection volume. The effluents were detected at a wavelength of 292 nm using the TUV detector. The method was validated concerning specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Tezacaftor and Ivacaftor were found to be 0.999 and 0.999, respectively. Recovery of Tezacaftor and Ivacaftor in the formulation was found to be 99.97% and 99.65% respectively.
Authors and Affiliations
Shyamala et al.
Keywords
Related Articles
- EP ID EP665527
- DOI 10.13040/IJPSR.0975-8232.10(11).4968-73
- Views 93
- Downloads 0
PHARMACEUTICAL VALIDATION & PROCESS CONCEPTUALISATION OF ANCIENT INDIAN CALCIUM PREPARATION: SHANKHA BHASMA
In the present study, the pharmaceutical and therapeutic dimensions of Shankha Bhasma (Classical Ayurvedic marine medicine) is explored as a substitute for calcium supplements available in the market. Shankha Bhasma was...
PHARMACOGNOSTIC AND PHYTOCHEMICAL INVESTIGATION OF POTENTIALLY IMPORTANT PLANTS OF WESTERN GHATS, INDIA
Objective: The Indian traditional system of medicine, namely Ayurveda and Siddha, are primarily based on the use of the whole plant or different parts of plant singly or in combination to make multifactorial formulations...
IDENTIFICATION AND QUANTIFICATION OF OLEANOLIC ACID FROM CYCLEA PELTATA BY HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) METHOD
To identify and quantify oleanolic acid from the roots of cyclea peltata by HPTLC method. Methods: The Plant cyclea peltata is a perennial herb from Menispermacae family. The powdered roots of plant cyclea peltata were e...
STUDY OF ANTI-LIPIDEMIC EFFECT OF LEMONGRASS (CYMBOPOGON CITRATUS) AQUEOUS ROOTS AND FLOWER EXTRACTS ON ALBINO MICE
Purpose/Aim: In the present study, we investigate the antilipidemic effect of the aqueous extract Cymbopogon citratus. Background: Lemongrass is an aromatic plant belonging to the Gramineae family. C. citratus has been c...
SYNTHESIS OF POTENTIAL ANTI-INFLAMMATORY PYRAZOLINE DERIVATIVES UNDER ULTRASONIC IRRADIATION
In the present investigation, the chalcone intermediates are synthesized by Claisen-Schmidt condensation reaction between acetanilide and appropriate aromatic aldehydes. Further, these chalcone intermediates were cyclize...