A study on genotypic frequency of 12-lipoxygenase polymorphisms and 12-HETE levels in type II diabetes
Journal Title: Medpulse International Journal of Biochemistry - Year 2018, Vol 7, Issue 3
Abstract
Background: Diabetes mellitus describes a group of metabolic diseases characterized by a progressive insulin secretory defect due to insulin resistance resulting in high glucose levels in the blood. Approximately 90% of all the cases of diabetes worldwide are type II Diabetes Mellitus where enough insulin is not produced for proper function. Growing body of evidence has suggested that beta cell inflammation and dysfunction play a key role in the pathogenesis of type II diabetes. Obesity and lack of exercise are some of the contributory factors for type II diabetes mellitus. A class of enzymes called lipoxygenases and derived oxidized lipids induces inflammation of beta cells and has been implicated in diabetes development. Studies show that the genetic deletion of 12-lipoxygenase (12-LOX) decreases the activity of 12-LOX and further decrease beta cell inflammation and dysfunction. Hence our main objective was to study the polymorphisms of 12-LOX gene in type II diabetes. Materials and Methods: Study participants included were in the age group of 30-70 years. 30 diagnosed patients of diabetes with the minimum history of 3 years were selected as cases. Equal number of age and sex matched healthy individuals were included in the control group. A detailed clinical history, family history and demographic details were collected from the subjects. Body Mass Index (BMI), HbA1c levels, Plasma 12-Hydroxyeicosatetraeinoic acid (12-HETE) levels and 12-LOX gene polymorphisms were assessed. Results and Conclusion: We observed significant difference in the routine parameters such as Fasting blood sugar (FBS), HbA1c and BMI whereas the 12-HETE levels did not show any significant difference between diabetic and non-diabetic subjects. Polymorphism study revealed varied genotype distribution of G>A and C>T gene clusters but again did not show any significance.
Authors and Affiliations
Suma M Nataraj, Devananda Devegowda, Anisha Mathai, Ankita Mysore Amarbabu, Arpitha Bankenhalli Jayaram, Pushkal Sinduvadi Ramesh, Swetha Kempegowda
Keywords
Related Articles
- EP ID EP442662
- DOI 10.26611/1002733
- Views 163
- Downloads 0
Correlation of 24-hour urinary protein, urinary protein: Creatinine ratio and HBA1c in type 2 diabetes with and without nephropathy
Background: The leading cause of morbidity and premature mortality in diabetics is nephropathy. closely associated with renal involvement in diabetes. Objectives: To estimate serum levels of HbA1c, 24 ratio in Type 2 dia...
Identification and corrective actions of preanalytical errors in clinical biochemistry laboratory of a pediatric tertiary care hospital: A two year study
Abstract Objective: To identify the leading causes of preanalytical errors in clinical biochemistry laboratory and suggesting few corrections. Methods: A retrospective analysis of the records and log registers for 2016-1...
Study of effects of Epigallocatectin-gallate (EGCG) on lipid profile and thereby cardiovascular risk
Background: Nowadays use of green tea has been increasing worldwide due to the beneficial effects it has on cardiovascular system. The beneficial effects of green tea can be attributed to the presence of various catectin...
Association of serum leptin and diastolic blood pressure in pre-eclamptic women
Background: Maternal peripheral leptin levels are enhanced during normal pregnancy and collectively suggest that leptin concentrations peak in the second trimester. A better understanding of leptin and its role in PE may...
Correlation of serum malondialdehyde level with components of metabolic syndrome
Background: Metabolic syndrome is one of the major public health concerns and is emerging as a pandemic. oxidative stress is a hallmark of metabolic syndrome and plays a central role in the progression of the disease. Ma...