A VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF BACLOFEN IN BULK DRUG AND PHARMACEUTICAL FORMULATIONS
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 2
Abstract
A new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed and validated for the estimation of Baclofen in bulk drug and pharmaceutical formulations. Baclofen was chromatographed on a reverse phase C18 column (150x4.6mm I.D., particle size 5 μm) in a mobile phase consisting of ammonium acetate buffer (pH 5.0 adjusted with orthophosphoric acid) and methanol in the ratio 40:60 v/v. The mobile phase was pumped at a flow rate of 1.0 mL/min with detection at 220 nm. The detector response was linear in the concentration of 10-50μg/mL. The limit of detection and limit of quantitation was found to be 1.025 and 3.107µg/mL, respectively. The intra day and inter day variation was found to be less than 1%. The mean recovery of the drug from the solution was 100.1%. The proposed method is simple, fast, accurate, precise and reproducible hence, it can be applied for routine quality control analysis of Baclofen in bulk drug and pharmaceutical formulations.
Authors and Affiliations
Rajesh M, Manzoor Ahmed, Maanasa Rajan BN
Keywords
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