Method development and validation for the estimation of Divalproex sodium by using RP-HPLC in bulk and pharmaceutical dosage forms
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2018, Vol 6, Issue 2
Abstract
New RP HPLC method was developed for the estimation of Divalproex Sodium pharmaceutical dosage form. Solubility of Divalproex sodium was determined using various solvents and buffers. Absorption maxima of the drug in UV–Visible region in different solvents/buffers was determined and different solvents were tried for HPLC method development. Mobile phase was optimized and flow rates for proper resolution and retention times. HPLC method was validated as per ICH guidelines.
Authors and Affiliations
M. Fatima Rose, Chattumala Anand Kumar, Injamuri Diwakar, Jangam Vanaja, Kottu Gayathri, Julakanti Likhitha, Hassan EaisaMohamed Hamid
Keywords
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- EP ID EP33511
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Method development and validation for the estimation of Divalproex sodium by using RP-HPLC in bulk and pharmaceutical dosage forms
New RP HPLC method was developed for the estimation of Divalproex Sodium pharmaceutical dosage form. Solubility of Divalproex sodium was determined using various solvents and buffers. Absorption maxima of the drug in U...