Ambiguities Resolution of HLA Genotypes in Macedonian Population
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2008, Vol 1, Issue 2
Abstract
A total of 850 persons from the Republic of Macedonia, divided in the four groups, were DNA-HLA genotyped: 1. Donors of the Macedonian Donor Registry (220); 2. Patients for bone marrow transplantation and related potential donors (350); 3. Patients with rheumatoid arthritis, tuberculosis, cardiomyopaties and other diseases (150); and 4. Ambiguity resolution of the genotypes (130). Class I HLA genes (A, C, B, and DQB1) were typed by Reverse Line Strip Typing (RLS); Class II HLA gene (DRB1) were typed by Sequence Based Typing (SBT). The main results of the project are: a) Creation and legislation of Macedonian Donor Registry, and Macedonian Human DNA Bank; b) HLA typing of unrelated healthy persons for class I HLAA, -C, and –B, and class II HLA-DRB1; c) HLA genotyping of blood related samples for Bone Marrow Transplantation; d) Association analysis of HLA alleles with Rheumatoid Arthritis (HLA-DRB1, and HLA-B27), and with Pulmonary Tuberculosis (HLA-DQB1); e) Evaluation of allele and genotype ambiguities for HLA class I, and HLA class II, as well as resolution of HLA class I and class II ambiguities; f) Introduction and establishment of sequence specific primers (SSP) method for resolving HLA class II ambiguities in patients with Rheumatoid Arthritis.
Authors and Affiliations
Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Aleksandar Petlichkovski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia, Ana Strezova| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss Kiril and Metodij”, Skopje, Republic of Macedonia
Keywords
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- EP ID EP8602
- DOI 10.3889/MJMS.1857-5773.2008.0008
- Views 324
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