An evaluation of using multiplex Real-Time PCR assay for the identification of factors causing hospital bacterial infections
Journal Title: JOURNAL OF FUNCTIONAL VENTILATION AND PULMONOLOGY - Year 2019, Vol 10, Issue 31
Abstract
Introduction. To develop a multiplex real-time PCR for simultaneous rapid detection causing nosocomial such as Staphylococcus aureus, Acinetobacter Baumannii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. Subjects and Methods. 143 samples were selected from 455 patients has been infected in the intensive care unit. The samples were divided into 2 parts, one of its cultured, other part used molecular biology technique compare the results obtained. The multiplex real-time PCR reaction have been optimized to ensure successful for amplification of the target genes. Sensitivity and consistency are based on culture results. Results. Multiplex real-time PCR assay were employed to detection bacterial: Staphylococcus Aureus, Acinetobacter Baumannii, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa.This had values compared culture method: sensitivity (97.6%), specificity (96.8%). Kappa coefficient shows that the two methods were very good similarities (ƙ value= 0.98, p <0.05). Conclusions. Multiplex real-time PCR was a Rapid, high sensitivity, specificity method to detection batteries.
Authors and Affiliations
TRAN THANH Tu
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- EP ID EP590931
- DOI 10.12699/jfvpulm.10.31.2019.15
- Views 90
- Downloads 0
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An evaluation of using multiplex Real-Time PCR assay for the identification of factors causing hospital bacterial infections
Introduction. To develop a multiplex real-time PCR for simultaneous rapid detection causing nosocomial such as Staphylococcus aureus, Acinetobacter Baumannii, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aerug...