Analytical method development and validation for the estimation of Olmesartan medoxomil by RP-UPLC in bulk and pharmaceutical dosage forms.
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
A simple rapid, accurate, precise and reproducible validated reverse phase UPLC method was developed for the determination of Olmesartan medoxomil in bulk and pharmaceutical dosage forms. The quantification was carried out using Waters acquity UPLC BEH C18 (100 X 2.1 mm, 1.7 µm) column run in Isocratic way using pH 3.4 Buffer: Acetonitrile (60:40% v/v)] and a detection wavelength of 250nm, and injection volume of 4µL, with a flow rate of 0.3mL/min. The retention times of Olmesartan medoxomil was found to be 3.418. The method was validated in terms of linearity, precision, accuracy, LOD, LOQ and robustness in accordance with ICH guidelines. The linearity ranges of the proposed method lies between 0.080 mg/mL to 0.120 mg/mL, which is equivalent to 10% to 150% and with correlation coefficient of r2=0.9999.The assay of the proposed method was found to be 98.79%. The recovery studies were also carried out and mean % Recovery was found to be 100.5%. The % RSD from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Olmesartan medoxomil in bulk and in Pharmaceutical dosage form.
Authors and Affiliations
Farhana Pattan, Haritha Pavani, Chandana N, Karimulla M
DEVELOPMENT AND VALIDATION OF A SIMPLE AND RAPID RP- HPLC METHOD FOR THE DETERMINATION OF METRONIDAZOLE AND NORFLOXACIN IN COMBINED DOSAGE FORM
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Metronidazole and Norfloxacin i...
Formulation and evaluation of Zolmitriptan controlled release matrix tablets
Controlled release formulations in many case provide significant advantages, including improved therapeutic effect, increased patient compliance by reducing dosing frequency and decrease in incidence and or intensity o...
RECENT TRENDS IN SCOPE AND OPPORTUNITY OF CLINICAL RESEARCH IN INDIA
Clinical Research is one of the most knowledge-intensive industry. It is complete biography of drug from its inception in the lab to its introduction to the consumer market and beyond. Any molecule is identified is sub...
Cardio protective activity of Passiflora foetida extract and silver nanoparticles in doxorubicin induced cardiac disease in rats
Medicinal plants have been observed to possess numerous activities with regard to cardiovascular system viz. antiplatelet, hypolipidemic, anti-inflammatory, hypoglycemic and hypotensive actions. For cardiovascular dise...
EFFECTIVE HYPOGLYCEMIC ACTION OF METFORMIN COMBINATIONS AGAINST DEXAMETHASONE INDUCED DIABETES MELLITUS IN RATS
Diabetes mellitus is a chronic metabolic disorder occurs due to the damage of β-cells of islets of Langerhans in pancreas. In general patients suffering for diabetes mellitus always use the medicines, apart from the me...