Analytical method development and validation for the estimation of Olmesartan medoxomil by RP-UPLC in bulk and pharmaceutical dosage forms.
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
A simple rapid, accurate, precise and reproducible validated reverse phase UPLC method was developed for the determination of Olmesartan medoxomil in bulk and pharmaceutical dosage forms. The quantification was carried out using Waters acquity UPLC BEH C18 (100 X 2.1 mm, 1.7 µm) column run in Isocratic way using pH 3.4 Buffer: Acetonitrile (60:40% v/v)] and a detection wavelength of 250nm, and injection volume of 4µL, with a flow rate of 0.3mL/min. The retention times of Olmesartan medoxomil was found to be 3.418. The method was validated in terms of linearity, precision, accuracy, LOD, LOQ and robustness in accordance with ICH guidelines. The linearity ranges of the proposed method lies between 0.080 mg/mL to 0.120 mg/mL, which is equivalent to 10% to 150% and with correlation coefficient of r2=0.9999.The assay of the proposed method was found to be 98.79%. The recovery studies were also carried out and mean % Recovery was found to be 100.5%. The % RSD from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Olmesartan medoxomil in bulk and in Pharmaceutical dosage form.
Authors and Affiliations
Farhana Pattan, Haritha Pavani, Chandana N, Karimulla M
Keywords
Related Articles
- EP ID EP33151
- DOI -
- Views 294
- Downloads 0
Health and oral health literacy – a review of literature
The process of acquiring health information, appraising its concepts, and applying health prevention and treatment plans appropriately requires new skill development called health literacy. Research in health literacy...
Biomedicine and biotechnology: applications and horizons in dentistry-a review
Biomedicine is a branch of medical science that applies biological and other natural-science principles to clinical practice whereas Biotechnology is the mere application of technical advances in life science to develop...
PREPARATION, CHARACTERIZATION AND EVALUATION OF OLMESARTAN MEDOXOMIL-Β CYCLODEXTRIN COMPLEXES
Olmesartan medoxomil is a poor water soluble drug. There are numerous approaches available and reported in literature to enhance the solubility of poor water soluble drugs among which cyclodextrin complexation is predo...
Preclinical evaluation of cardio protective activity of Grewia hirsuta on doxorubicin induced cardiotoxicity in rats
In this study, an attempt was made to investigate preclinical evaluation of cardio protective activity of Grewiahirsuta induced cardio toxicity in rats. The phytochemical screening was done for the selected plant. The...
Formulation and evaluation of colon targeted Aceclofenac tablets
Aceclofenac colon targeted tablets were prepared by using natural polymers like guar gum and Locust bean gum. Matrix core tablets were prepared by using various concentrations of polymers. The core tablets were coated...