Analytical method development and validation for the simultaneous estimation of Levamisole and Mebendazole in bulk & tablet formulation by RP-HPLC method
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2014, Vol 2, Issue 1
Abstract
A fast, robust and accurate RP-HPLC method was developed and validated for simultaneous determination of Levamisole and Mebendazole in tablets. The mobile phase was mixture of aqueous phosphate buffer with pH 5.2: Methanol: Acetonitrile (30:20:50 v/v/v), effluent flow rate monitored at 1.0 ml/min. the stationary phase was C18 column, zodiac 5µ (4.6×250mm). The solutions of standard and the sample were prepared in methanol. The retention times was found to be 2.427min and 4.110min for Levamisole and Mebendazole respectively at 235nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of 150-210µg/ml. The percentage assay for Levamisole and Mebendazole were found to be 99.94% and 99.35%respectively. The LOD and LOQ were found to be0.0034µgm/ml and 0.00104µgm/ml for Levamisole, 90-210µgm/ml and 60-140µgm/ml for Mebendazole. The method was validated and it was found to be accurate, precise, linear and reproducible.
Authors and Affiliations
V. Rama Koteswara Rao, Nanda Kishore Agarwal, K. Haritha Pavami, B. Prem Kumar, R. Mallikarjuna
Keywords
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