Anti-Diabetic activity of hydro-alcoholic root extract of Echinops echinatus and its beneficial effects on nephropathy in experimental rats
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 1
Abstract
Anti-diabetic activity of 70% Hydro-alcoholic root extract of Echinops echinatus at a dose of (100& 200 mg/kg body weight p.o) was investigated in diabetes Nephropathic rats. α- Amylase inhibitory activity of the Echinops echinatus was determined against Diastase and has shown 72% inhibitory effect of amylase at conc of 25 mg/ml. The preliminary phytochemical screening of hydro-alcoholic root extract revealed the presence of alkaloids, carbohydrates, steroids, glycosides, flavonoids, phenols, tannins, aminoacids and absence of saponins and terpenoids. In acute toxicity study (423 Guideline), there was no mortality up to a dose of 2000mg/kg body weight p.o, thus considered as maximum tolerated dose. When treated with 70% Hydro-alcoholic root extract of Echinops echinatus at a dose of 100 and 200 mg/kg b.w p.o. Group IV and group V has produced significant regeneration of cells in diabetic nephropathic rats under histopathological studies. The effect was more pronounced in 200 mg/kg b.w than 100mg/kg body weight & showed Nephroprotective as obvious by significant reinstatement levels of Oral glucose tolerance test, blood glucose levels in biological parameters serum urea, serum creatinine &serum lipid profile (Triglycerides, Total cholesterol, HDL, LDL, VLDL)The purpose of the study was to evaluate the antidiabetic activity of hydroalcoholic extract on kidneys in experimental rats by using α- amylase & Alloxan induced diabetic rats. Results showed that the 70% Hydro-alcoholic root extract of Echinops Echinatus exhibited significant and dose-dependent Anti-diabetic activity in all the diabetic common rats. Annova tests for biological parameters(blood glucose level, Serum urea, serum creatinine, HDL, LDL, VLDL) was performed by using Dunnet's t- tests and was found to be significant p<0.0001; p<0.01; p<0.05. Results of our study suggest that 70% Hydro-alcoholic root extract of Echinops echinatus possess Anti-diabetic activity which may be due to the presence of flavonoids in the extract as it has anti-secretory, cytoprotective and antioxidant properties, antidiabetic, anti-hyperglycemic, anti-inflammatory, hypoglycemic, diuretic, anti-bacterial, antifungal, antispasmodic, antipyretic and analgesic.
Authors and Affiliations
Sumia Fatima, Sameera Afroz, Abdul Saheel Qureshi
Keywords
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