ANTIBACTERIAL ACTIVITY OF STEM AND ROOT EXTRACTS OF Avicennia officinalis L
Journal Title: International Journal of Pharmaceutical Applications - Year 2011, Vol 2, Issue 4
Abstract
Extracts of stem and root of Avicennia officinalis L. were prepared in hexane, benzene, ethyl acetate, acetone, methanol and ethanol, and their antibacterial activity was evaluated against Escherichia coli MTCC 64, Enterobacter aerogenes MTCC 111, Klebsiella pneumoniae MTCC 39, Pseudomonas aeruginosa MTCC 424, Bacillus subtilis MTCC 121, Lactobacillus delbrueckii MTCC 911, Staphylococcus aureus MTCC 87 and Streptococcus pyogenes MTCC 1928 by agar well diffusion method. The stem and root extracts in acetone demonstrated best antibacterial activity. However, extracts prepared in other solvents also showed antibacterial activity against the test organisms. The extracts that possessed antibacterial activity were further used for the determination of the Minimum Inhibitory Concentration (MIC) taking different concentrations viz., 1.25, 2.5 and 5.0mg /100μl . The value of MIC was found to be ranged from 1.25mg/100μl to 5mg/100μl for different test organisms. Further, the extracts that demonstrated the antibacterial activity were checked for their bactericidal and / or bacteriostatic nature. The present study reveals the potential of stem and root extracts of Avicennia officinalis L. as antibacterial agent.
Authors and Affiliations
N. Sharief Md , UmaMaheswara Rao V
Keywords
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