Antimicrobial Potential and In Vitro Cytotoxicity Study of Tabernaemontana Divaricata (L.) Stem Bark Extract Against HEK 293 Cell Line
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 3
Abstract
Resistance of antibiotics against pathogenic microbes is a growing clinical concern, and the spread of resistant strains worldwide has been discombobulating public health. The ability of most infectious microbes evolving antimicrobial resistance necessitates discovery of novel antimicrobial agents from natural sources. The present study was focused on identification of multi drug resistant (MDR) microorganisms of clinical isolates and that were subjected to methanolic extracts of Tabernaemontana divaricata stem bark crude extract for antimicrobial efficacy by agar well diffusion method. Phytochemical analysis and the minimum inhibitory concentration (MIC) testing were also determined. In vitro cytotoxic effects of the stem bark extract was observed through MTT reduction assay on normal cell line. In our study we have found antimicrobial activity against all test isolates by agar well diffusion methods. The less MIC value of methanolic extract was profound against MRSA as compared to other isolates by microtitre plate-based assay. GC-MS analysis revealed that the presence of two most probable active antimicrobial compounds viz. cyclotetrasiloxaneoctamethyl and cyclopentasiloxanedecamethyl respectively. To our knowledge, this is the first report of the presence of the said compounds with antimicrobial activity in T.divaricata. We have also evaluated possible cytotoxic effect on Normal cell lines (IC50= 56.52μg/mL). Our study suggests the possibility of exploring antimicrobial property in the active compound of Tabernaemontana divaricata as a prospective alternative therapy that could help in combating growing antibiotic resistance trend worldwide. So, in traditional medicine our investigation provides a good insight for drug development process and clinical practices.
Authors and Affiliations
Mrinal Kumar Baishya, Kandarpa Kr. Saikia1, Naba Kr. Hazarika, Baishya1 Deep Jyoti Das
Keywords
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