Antioxidant Properties and Alpha-Amylase Inhibition of Wild Blackberry Leaf
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
In this study, the possible antioxidant properties of ethanolic extract of wild blackberry leaves were investigated by using antioxidant assays including DPPH• scavenging and β-carotene bleaching method. The leaf extract was showed DPPH radical scavenging activity at the 100 µg/mL and above concentrations and inhibited lipid peroxidation at the high concentrations. The natural compounds known as potential antioxidants such as phenolics, flavonoids were also determined. Total phenolic content was determined using FolinCiocalteu method and was found to be 186.05±1.46 µg of gallic acid equivalents. Total flavonoid content was studied using AlCl3 reagent and was determined as 19.39±0.83 µg of quercetin equivalents. The different concentrations of leaf extract had α-amylase inhibitory activity in the range of 21 - 44 %. The observed inhibition effect against α-amylase could be due to phenolic contents of the plant. In conclusion, the leaves of wild blackberry may be used as a natural antioxidant source in both food and nutritional applications.
Authors and Affiliations
Sebnem Selen Isbilir, Hulya Yagar
Keywords
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- EP ID EP386771
- DOI 10.9790/3008-1106030609
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