ANTIOXIDANT PROPERTIES OF NATURAL POLYPHENOLIC EXTRACTS FROM SELECTED PLANTS IN MODEL SYSTEMS
Journal Title: Żywność. Nauka. Technologia. Jakość - Year 2012, Vol 19, Issue 6
Abstract
The objective of the study was to determine the antioxidant properties of selected plant extracts from St John’s wort, buckwheat, hawthorn, helichrysum, as well as their important polyphenol constituents towards phosphatitylcholine liposome membranes (PC) oxidized with UVC radiation. Also, there were determined the antiradical properties of those substances towards the free radical 2,2-diphenyl-1- pikrylhydrazyl (DPPH•) and the membrane association constants using a DPH (l,6-diphenyl-l,3,5- hexatrien) probe. There was confirmed the following relation of the antioxidant activities of the extracts, expressed as an IC50 PC parameter: hawthorn – bark (21.69 mg/l) > St John’s wort (25.45 mg/l) > buckwheat – hull (30.33 mg/l) > hawthorn – leaves (32.52 mg/l) > buckwheat – haulm (37.47 mg/l) > helichrysum (115.06 mg/l). The antioxidant activity of selected polyphenol components changed as follows: quercetin (0.53 mg/l) > epicatechin (29.19 mg/l) > chlorogenic acid (62.59 mg/l) > rutin (78.97 mg/l). The positive correlation between the antioxidant activity of the extracts studied and phenolic compounds, and the antiradical activity (r≥0.94) indicates that the mechanism of their antioxidant activity consists in neutralizing free radicals. From among the flavonoids studied, the quercetin showed the highest antioxidant activity, a high antiradical activity (IC50 DPPH• = 2.51 mg/l), and a high association constant towards the liposome membrane (Ka = 11.23 103 l/mg). The quercetin molecules may deeply anchor into the liposome membrane, therefore, free radicals are obstructed when penetrating into the inside of the membrane; for that reason, the antioxidant effectiveness of the liposome membrane is enhanced.
Authors and Affiliations
Kamil Sierżant, Katarzyna Pyrkosz-Biardzka, Janina Gabrielska
Keywords
Related Articles
- EP ID EP110732
- DOI 10.15193/zntj/2012/85/041-053
- Views 80
- Downloads 0
ZIARNO ZBÓŻ I PRODUKTY ZBOŻOWE JAKO ŹRÓDŁA BŁONNIKA POKARMOWEGO
Na podstawie badań interdyscyplinarnych wykazano ścisły związek między poziomem spożycia błonnika pokarmowego a liczbą zachorowań na przewlekłe choroby niezakaźne. W pracy omówiono definicję błonnika pokarmowego oraz met...
Ocena możliwości stosowania dozwolonych oświadczeń żywieniowych i zdrowotnych dotyczących kwasów tłuszczowych w odniesieniu do orzechów jadalnych
W pracy oznaczono i porównano zawartość oraz profil kwasów tłuszczowych lipidów różnych orzechów jadalnych, jak również oceniono ich przydatność żywieniową w kontekście stosowania dozwolonych oświadczeń żywieniowych i zd...
ANTIOXIDANT PROPERTIES OF BLACK ELDER FLOWERS AND BERRIES HARVESTED FROM THE WILD
In the paper, there were presented the results of the research into antioxidant properties of aqueous extracts of black elder flowers and berries harvested in 17 wild sites in Poland, and the effect of total polyp...
SUGAR COMPOSITION OF APPLE CULTIVARS AND ITS RELATIONSHIP TO SENSORY EVALUATION
PODSTAWOWY SKŁAD CHEMICZNY ORAZ AKTYWNOŚĆ PRZECIWRODNIKOWA ZIELA WYBRANYCH GENOTYPÓW OWSA SIEWNEGO (AVENA SATIVA)