Assessment of protein carbonyl in type II diabetes mellitus in a tertiary care hospital

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Assessment of protein carbonyl in type II diabetes mellitus in a tertiary care hospital

Journal Title: Medpulse International Journal of Biochemistry - Year 2019, Vol 10, Issue 2

Abstract

Background: Diabetes mellitus (DM) is an endocrine dysfunction, which is related with metabolic disorder. Hyperglycemia occurs primarily due to the insufficiency of secretion or action of endogenous insulin. It is accelerated by reduced glycemic control which increases free radicals causing oxidative stress. Materialsand Methods: Overall 180 subjects were involved in the study. The cases comprised of 45 each of male female diabetics. 45 non diabetic males and 45 non diabetic females were selected as controls. Protein carbonylation in T2DM (Type 2 diabetes mellitus) subjects was assessed by DNPH method. In our study the mean and SD values of protein carbonyl were considered for all cases and control of both sexes of three different age groups. Results: Both diabetic males and females had elevated Protein carbonyl(PC) level as compared to controls (p value 0.05). The present study shows that with raise in free radicals in T2DM causes oxidation of protein which in term increases a level of PC and also support an involvement between these protein oxidation and type 2 diabetes as well as their positive association with different age groups The stronger response observed in serum samples from patients with higher patients’ age groups compared with lower age groups. Conclusion: our findings suggest that protein carbonyl contents may be useful in evaluating the progression of diabetes mellitus type 2 and in elucidating the mechanisms of disease pathogenesis

Authors and Affiliations

GRavi kumar, SHari Priya, Nagarjuna Sivaraj

Keywords

diabetes mellitus

EP ID EP613231
DOI 10.26611/10021022
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