Biologically-Induced Precipitation of Minerals in a Medium with Zinc Under Sulfate-Reducing Conditions
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 2
Abstract
[b][/b]Sulfate-reducing microbial communities were enriched from soils collected in areas with crude-oil exploitation. Cultures were grown in modified Postgate C medium and minimal medium, with ethanol or lactate as an electron donor. The batch cultures were grown with addition of zinc in concentrations of 100–700 mg/l. A lack of increased protein concentration in the solutions compared with the control batch, was noted in cultures containing over 200 mg Zn[sup]2+[/sup]/l. The 16S rRNA method was applied to determine the specific composition of the selected microorganism communities. The analysis indicated the presence of [i]Desulfovibrio[/i] spp., [i]Desulfobulbus[/i] spp. and [i]Desulfotomaculum[/i] spp. in the communities. Diffractometric analysis indicated the presence of biogenic sphalerite in cultures with 100 and 200 mg Zn[sup]2+[/sup]/l and elemental sulfur in cultures with 200 mg Zn[sup]2+[/sup]/l. Other post culture sediments (300−700 mg Zn[sup]2+[/sup]/l) contained only hopeite [Zn[sub]3[/sub](PO[sub]4[/sub])[sub]2[/sub] [sup].[/sup] 4H[sub]2[/sub]O] formed abiotically during the experiment, which was confirmed by studies of the activity of sulfate-reducing microbial communities.
Authors and Affiliations
Dorota Wolicka, Andrzej Borkowski, Urszula Jankiewicz, Paweł Kowalczyk, Wojciech Stępień
Keywords
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