CFIA- Colorimetric assessment and photometric determination of vitamin B9 (Folic acid)using LEDs as a source of irradiation and two solar cells as an energy transducer
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
A specific, new, sensitive and simple method has been used for the determination of vitamin B9(Folic acid )in pure and pharmaceutical formulations by continuous flow injection analysis .The method is based on oxidation vitamin B9 by Ce(IV) sulphate in sulphuric acid to obtain a red complex, using homemadeAyah-6SX1- ST-2D solar cell CFIA. Optimum parameters have been studied to increase the sensitivity and limit of detection for this developed method. The linear range for the instrument response versus vitamin B9 concentration was (0.1-5) mMol.L-1 while the L.O.D was 5.544µg/sample, the correlation coefficient ( r ) was 0.9953 while percentage linearity ( r2% ) was 99.07%. RSD% for the repeatability (n=8) was less than 0.5%for the determination of vitamin B9 at concentration (0.9, 5) mMol.L-1 respectively .The method was applied successfully for the determination of vitamin B9 in pharmaceutical preparation .A comparison was made between two methods: newly proposed method and the reference claimed method UV-SP spectrophotometry at ʎmax=255 nm, of analysis using the standard addition method, via the use paired t-test and there was no significant between the two methods at 95% confidence level.
Authors and Affiliations
Proph. Nagam S. Turkey Al-Awadi, Rana A. Kamal Aldeen
Keywords
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- EP ID EP391882
- DOI 10.9790/3008-1205071022
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