Combination of Ficus Carica Leaves Extract and Ubiquinone in a Chronic Model of Lithium Induce Reproductive Toxicity in Rats: Hindrance of Oxidative Stress and Apoptotic Marker of Sperm Cell Degradation
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 2
Abstract
Lithium carbonate (LC) is the most widely used as antipsychotic drug and can suppress reproductive performance via testicular toxicity and induction of oxidative stress. The aim of the study was to investigate biological activities of Ficus carica extract (FC) and ubiquinone (Coq10) against LC induced reproductive toxicity in male rats. For this purpose forty two Wistar albino rats were used in this study and allocated into seven groups 6 of each. 1 st groups was served as normal control rats, received distill water (C). 2nd groups orally treated with 200 mg /kg body weight of Ficus carica extract (FE). 3rd groups orally administered with 20 mg /kg body weight of CoQ10 (CoQ10). 4th groups orally treated with 25 mg /kg body weight of Lithium carbonate (LC). 5th group simultaneously treated with 25 mg /kg body weight of Lithium carbonate and 200mg/kg b.w of FE (FC+ LC). .6th group simultaneously treated with 25 mg /kg body weight of Lithium carbonate and CoQ10 (20 mg/kg b.w) (LC+CoQ10). 7th groups treated with 25 mg /kg body weight of Lithium carbonate, FE (200mg/kg b.w) and CoQ10 (20 mg/kg b.w) (FC+CoQ10+LC). The experiment lasted for 60 days. Revealed data showed that the LC induces significant (P>0.05) increase in serum ALT, AST level, testicular GSSG, NO, MDA and 8-hydroxy-2-deoxyguanosine (8OHdG). But showed significant (P>0.05) decrease in testicular GSH, endogenous antioxidant enzymes (SOD and CAT), cell energy (ATP), testosterone level and semen quality [Sperm cell concentration (SCC), mass motility (MM) and individual motility % (IM)]. On the other hand, FC, CoQ10 and their combination ameliorate negative impacts exhibited by LC. Data was confirmed by histological examination and Immunohistochemical staining for caspase-3 (as proapoptotic marker) of testes which revealed pathological alteration in LC, significant recovery in FC, CoQ10 and FC+CoQ10+LC. In conclusion, the present study suggests that FC, CoQ10 and their combination ameliorate LC-induced reduction in reproductive performance in male rats.
Authors and Affiliations
1 Shimaa A. Haredy, 2 Tamer S. Imam, 1Omar A. Ahmed-Farid
Keywords
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- EP ID EP388550
- DOI 10.9790/3008-1202026473
- Views 75
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