Comparison of estrogen receptor, progesterone receptor and Ki-67 immunohistochemistry scoring using manual method and image analysis in breast carcinoma cases
Journal Title: Medpulse International Journal of Pathology - Year 2018, Vol 6, Issue 1
Abstract
Background: Breast carcinoma is the leading cause of carcinoma death in women. Estrogen receptors(ER), progesterone receptors(PR) and Ki-67 are important prognostic determinators. However immunohistochemistry (IHC) reporting always has a degree of subjectivity and interobserver variability. This study was done to validate digital image analysis of IHC images using Immunoratio web plugin and if it can overcome the above difficulties. Objectives: To score ER, PR and Ki 67 immunostaining by manual light microscopy and image analysis software. To compare the scoring done by manual method versus image analysis. Methods: 50 breast carcinoma IHC slides each of ER, PR and Ki67 performed at the Department of Pathology, Sri Devaraj URS Medical College from year 2009- 2014 was be taken up for study. IHC was done with Biogenex Antibody using standard IHC protocol. The IHC slides were reported by two pathologists and micrograph of 3 select areas were analysed using Immunoratio image analysis software and the results were compared. Analysis software uses multistep process for quantitative analysis and colour histogram module for intensity analysis. Results: IHC image analysis had a sensitivity and specificity of more than 90% in scoring ER and PR as positive and negative using Allred Score. However with Ki 67 the sensitivity was more than 80% only when the cut off was selected as 20%. Conclusion: Immunoratio image analysis software can be used to assist the pathologist in scoring IHC slides in reducing the subjective error rather than completely replacing manual scoring of IHC images. It will continue to be a good alternative to whole slide imaging with image analysis software in developing countries.
Authors and Affiliations
Swaroop Raj B V, Harendra Kumar M L
Keywords
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- EP ID EP460888
- DOI 10.26611/105614
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