Comparison of major peanut allergens Ara h 1, Ara h 2 and Ara h 3 between peanut cultivars from Côte d’Ivoire and the USA using SDS PAGE and Western Blots
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 1
Abstract
Peanut is commonly consumed in many forms. The ubiquitous presence of peanut in processed food is responsible for an increasing number of allergic reactions due to accidental ingestion. The prevalence of peanut allergy seems to be underestimated in the African population possibly because of the lack of testing and clinical documentation. In this study, a comparison was made between raw and roasted peanut seeds from cultivars of Côte d’Ivoire (ARA-CI) and raw peanut seeds from the cultivar Georgia Green, grown commercially in the USA. The main objective of this study was to identify the protein profile of peanut seeds from Côte d’Ivoire and compare it with the molecular specificities of major allergens of Georgia green seeds from the USA using a combination of two methods, SDS PAGE and Western blots. Peanut protein profiles via SDS PAGE, coupled with Western blots were carried out on two collections of peanut seeds. In the raw peanut seed extracts from Côte d’Ivoire, are visible fingerprints of the major allergenic proteins Ara h 1(63.5 kDa),Ara h 2(17, 20 kDa), and Ara h 3(25,36, 40 and 44 kDa) and an allergenic bands of Ara h 3 of about 36kDa.This provides evidence of the presence of the major allergens in peanut from Côte d’Ivoire, this, a presumption of a high allergenic potency peanut despite a low prevalence of peanut allergy in the country. The presence of a strongly expressed 30 kDa protein, potentially corresponding to a component of Ara h 3 in the roasted sample means that cooking processes could increase the allergenic potency of peanut. This study makes it possible to identify molecular specificity in peanut from Côte d’Ivoire for the development of local screening test adapted to the environment.
Authors and Affiliations
Chiayé Claire Antoinette Yapo-Crezoit, Antony Ananga 2 ,, Francis Yapo, Koffi Konan, Hortense Dodo
Keywords
Related Articles
- EP ID EP387308
- DOI 10.9790/3008-1201013136 .
- Views 86
- Downloads 0
In Vivo Anti-Trypanosomal Potential Of Methanol Root Extract Of Terminalia Macroptera (Guill. And Perr.) In Trypanosoma Brucei Brucei Infected Wistar Rat
Trypanosoma brucei brucei is a haemo-protozoan parasite transmitted by tsetse fly, which causes African animal trypanosomiasis (AAT), a parasitic disease that causes economic loss in cattle, sheep and goats. Existing dru...
Plate Tectonics and Reality
.
Antimicrobial Activity of Bauhinia Purpurea (L) by Minimum Inhibitory Concentration (MIC) Method
MIC methods are widely used in the comparative testing of new agents, or when a more accurate result is required for clinical management. As there are no CLSI (formerly NCCLS) recommendations for the determination of MIC...
Anti-Cancer Therapy: Chlorogenic Acid, Gallic Acid and Ellagic Acid in Synergism
Phytochemicals have been used as effective agents for treating illnesses. Its use has been widely increased in the present scenario where commercial drugs are carrying a huge burden of side effects. Many phytochemicals h...
Immunomodulatory Potential of Gemmomodified Extract of Terminalia arjuna
.In recent years there is increasing interest in the field ofmodulation of immune by plant products. The aim of this study was to evaluate the immunomodulatory potential of bark and gemmomodified extracts of Terminalia a...