Curcumin-Loaded Niosome Particle-Induced Cell Cycle Arrest and Apoptosis in Brain Cancer Stem Cell
Journal Title: The 1st Annual Meeting of Georgian Center for Neuroscience Research - Year 2020, Vol 2, Issue 20
Abstract
Background: Using a novel curcumin-loaded niosome particle (CM-NP), the present study was designed to evaluate the effect of curcumin on human glioblastoma stem-like cells (GSCs). CM-NP has a diameter of ~60 nm and a zeta potential of ~-35 mV with a constant physicochemical stability. The cytotoxic effects of free curcumin and CM-NP were investigated on GSCs obtained during the removal of a brain tumor. CM-NP caused a dose-dependent decrease in cell proliferation and viability of GSCs. The inhibition of cell viability was associated with the induction of apoptosis and SUB-G1 cell-cycle arrest in a p53-independent manner. CM-NP significantly increased the expression of Bax, a pro-apoptotic marker, and decreased the expression of Bcl2, an anti-apoptotic marker. CM-NP increased reactive oxygen species levels and decreased the mRNA expressions of NF-κB and IL-6. Methods: The quantity of apoptosis induced by curcumin and CM-NP against GSCs cells was measured via visualizing and quantitating the Bax, Bcl-2 gene by the RT-PCR assay. Results: The anti-proliferative activity of CM-NP in GSCs was associated with increased levels of reactive oxygen species and reduced expression levels of NF-κB and IL-6. Conclusion: Curcumin and CM-NP significantly reduced the expression of cancer stem cell markers, nestin and Sox2, in GSCs. These data indicate that CM-NP efficiently reduced the viability and proliferation of GSCs. This suggests CM-NP as an ideal carrier to deliver curcumin for potential therapeutic approaches in glioblastoma.
Authors and Affiliations
Fatemeh Ariakia, Sahar Salehi, Ghadir Rajabzadeh, Sajad Sahab-Negah, Ali Gorji
Keywords
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- EP ID EP677920
- DOI 10.29088/GCNR-2020.11
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