Detection of Methicillin Resistance and Vancomycin Resistance among Clinical Isolates of Staphylococcus aureus in a Tertiary Care Hospital at Tirupati
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2016, Vol 4, Issue 7
Abstract
Staphylococcus aureus is one of the important causes of community acquired and hospital acquired infections worldwide. Methicillin resistant Staphylococcus aureus is one of the major antibiotic resistant organisms. The present study was undertaken to determine the antibiotic sensitivity of Staphylococcus aureusisolates from various clinical samples and to evaluate the possible presence of MRSA, VISA (vancomycin intermediate Staphylococcus aureus) and VRSA (vancomycin resistant Staphylococcus aureus). A total of 120 isolates of S. aureus which were isolated from various clinical samples were tested for methicillin resistance using the oxacillin disc diffusion test (1µg) and cefoxitin disc diffusion test (30µg). All isolates were subjected to minimum inhibitory concentration (MIC) testing with agar dilution method according to the CLSI (Clinical Laboratory Standards Institutes) guidelines. Disc diffusion method was also used to determine the susceptibility of strains to common antibiotics. 64(53.3%) isolates were found to be MRSA by the cefoxitin disc diffusion method and 63(52.5%) isolates were found to be MRSA by oxacillin disc diffusion method. No VISA&VRSA isolates were detected by using the MIC agar dilution method. MRSA isolates were also highly resistant to other antibiotics that were tested. Keywords:Staphylococcus aureus, MRSA, Vancomycin Resistance
Authors and Affiliations
Dr. N. Ramakrishna
Keywords
Related Articles
- EP ID EP371291
- DOI -
- Views 69
- Downloads 0
Interest of tranexamic acid in total hip arthroplasty
Abstract: Total hip arthroplasty (THA) is one of the most frequent interventions in orthopedics. This is a surgical procedure at high risk of bleeding. Tranexamic acid (ATX), a synthetic antifibrinolytic, is an option of...
Detection of Methicillin Resistant Staphylococcus aureus in Various Clinical Samples Isolated from a Teaching Hospital
Abstract: Methicillin resistant Staphylococcus aureus (MRSA) became a major health care problem. Early and accurate detection of MRSA is essential for implementation of infection control practices.The resistance mechanis...
Prevalence of H. pylori Infection and Atrophic Gastritis among dyspeptic subjects in Cameroon using a Panel of Serum Biomarkers (PGI,PGII, G-17, HpIgG)
Abstract: H. pylori infection induces chronic active gastritis that develops with time in a proportion of infected people to atrophic gastritis (AG) and acid free or hypochlorhydric stomach.Objective: Use of a non-invasi...
Hepatoprotective activity of aqueous extract of Cynodon dactylon on paracetamol induced hepatotoxic albino rats
Abstract: Cynodon dactylon is commonly known as Bermuda grass. It is a perennial grass and found all over the India. The plant is a rich source of metabolites such as proteins, carbohydrates, minerals, β-sitosterols, fla...
The Prevalence of Salmonella typhi Carriers in Calabar Municipality of Cross River State, Nigeria.
This study was conducted to determine the prevalence of Salmonella typhi in Calabar Municipality. One thousand five hundred (1500) stool specimens were collected from food handlers, i.e. food-hawkers, canteen workers, re...