DETERMINATION OF DEGREE OF DEACETYLATION OF CHITOSAN - COMPARISION OF METHODS
Journal Title: Progress on Chemistry and Application of Chitin and its Derivatives - Year 2012, Vol 0, Issue
Abstract
Degree of deacetylation (DD) is one of the main parameters characterizing chitosan. The most precise measurements of DD require sophisticated equipment (NMR spectrometer), not available at many laboratories worldwide working on chitosan. There is a need for low-cost, simple, yet sturdy and reliable methods and procedures for DD determination. The aim of this work was to test and compare – on the same set of chitosan samples - a few of the existing analytical techniques and provide recommendation for selecting DD determination methods. Tests were performed on four chitosans of nominal DD in the range 70 - 95%. Three different methods of titration, two different methods of spectroscopy UV / Vis and infrared spectroscopy using various calculation approaches were used. The results are summarized and compared with values obtained by 1H NMR, considered as the reference method. Moreover, evaluation of the ease of performance and availability of reagents in the given methods was performed. On that basis, recommendations for selection of DD determination methods were formulated.
Authors and Affiliations
Renata Czechowska-Biskup, Diana Jarosińska, Bożena Rokita, Piotr Ulański, Janusz M. Rosiak
Keywords
Related Articles
- EP ID EP73548
- DOI -
- Views 93
- Downloads 0
INFLUENCE OF A PECTIN ON THE PROPERTIES OF HYDROPHILIC POWDERS CONTAINING A LACTIC ACID-CHITOSAN COMPLEX
Gynecological powders are designed to correct abnormal pH environments of the vagina and the stabilise the pH to the physiological state 3.5-5.0. Powders were transformed into gels and were tested for their properties. F...
INFLUENCE OF POLOXAMER 407 ON THE PROPERTIES GYNECOLOGICAL POWDERS CONTAINING LACTIC ACID COMPLEXED WITH CHITOSAN
Thermosensitive hydrophilic powders passing in gels containing lactic acid complexed with chitosan at a stoichiometric ratio 1:1 and 8:1 reveal pH ranged from 4.00 to 4.91 and 2.56 to 3.42. Rheological studies demonstrat...
PROCEDURE FOR DETERMINATION OF THE MOLECULAR WEIGHT OF CHITOSAN BY VISCOMETRY
The aim of this publication is to facilitate the estimation of chitosan molecular weight (MW) in laboratories with no access to sophisticated analytical instruments, by applying the easily accessible and economical capil...
SURFACE PROPERTIES OF CHITOSAN COMPOSITES WITH POLY(VINYL ALCOHOL) AND HYDROXYAPATITE
Surface properties of composites containing chitosan (Ch), poly(vinyl alcohol) (PVA) with hydroxyapatite (HAP) were investigated by contact angle measurements and atomic force microscopy (AFM). Thin films of Ch/HAP and C...
THE EFFECT OF PHYSICOCHEMICAL FACTORS ON ABSORPTION PROPERTIES OF CERTAIN SPASMOLYTICS IN THE PRESENCE OF DIETARY SUPPLEMENTS CONTAINING CHITOSAN
Chitosan is used in the treatment of obesity due to its lipid, cholesterol, fatty acids, triglycerides, bile acids binding capacity. A molecule of a cationic polymer, such as chitosan, is capable of binding acid drugs. T...