DETERMINATION OF THE HYDROLYTIC ACTIVITY OF WHOLE SALIVA USING CHITOSAN ASCORBATE AS A SUBSTRATE

Abstract

The aim of this work was to evaluate of hydrolytic activity of whole saliva using chitosan ascorbate as a substrate. In this aim, the concentrations of N-acetyl-D-glucosamine were determined in saliva before addition of chitosan ascorbate, directly after addition and during incubation with chitosan ascorbate by 20 hrs. In this in vitro study were used sterile chitosan ascorbate in the form of powder. Chitosan was obtained from krill chitin. The ratio of ascorbic acid to chitosan was 1:1. The unstimulated whole saliva showed the hydrolytic activity in the presence of the chitosan ascorbate as a substrate

Authors and Affiliations

Barbara Kochańska, Mirela Łukaszewska, Jolanta Ochocińska

Keywords

Related Articles

LARGE SIZE DNA IN VITRO AND IN VIVO DELIVERY USING CHITOSAN TRANSFECTION

In modern medicine, many diseases can be treated using gene therapy, which requires a DNA delivery system to prevent DNA from degradation and to transport it to the cells. Liposomal reagents are expensive for such a ther...

Use of Chitosan Film Coatings in the Storage of Carrots (Daucus carota)

Chitosan film applied to coat carrot slices affected metabolic changes that increased the content of total phenolic acids and flavonoids. Chitosan coating of carrot slices delayed the overripening process and, consequent...

HIGHLY SENSITIVE CHITOSAN-BASED OPTICAL FLUORESCENT SENSOR FOR GASEOUS METHYLAMINE DETECTION

An optical fluorescent sensor based on a chitosan thin film co-doped with Eu3+ and a bromothymol blue pH indicator has been developed. Near-UV to visible (350–400 nm) excitation of the europium (III) chelate complexes wi...

PHOSPHATE REMOVAL FROM AQUEOUS SOLUTIONS BY CHITIN AND CHITOSAN IN FLAKES

The article presents the effectiveness of phosphate adsorption on the flakes of chitin and chitosan. Studies performed determined adsorption capacity of chitin and chitosan, best among the tested adsorption pH and the eq...

INFLUENCE OF A CHOSEN AUXILIARY SUBSTANCE ON THE PROPERTIES OF HYDROPHILIC GELS CONTAINING LACTIC ACID COMPLEX WITH CHITOSAN

The wide range of pH and viscosity of the investigated preparations shall enable selection of a gel with optimum pH and rheological properties. The value of pH of a gel have swollen is greatly influenced by the content o...

Download PDF file
  • EP ID EP534736
  • DOI 10.15259/PCACD.23.09
  • Views 66
  • Downloads 0

How To Cite

Barbara Kochańska, Mirela Łukaszewska, Jolanta Ochocińska (2018). DETERMINATION OF THE HYDROLYTIC ACTIVITY OF WHOLE SALIVA USING CHITOSAN ASCORBATE AS A SUBSTRATE. Progress on Chemistry and Application of Chitin and its Derivatives, 0(), 97-102. https://europub.co.uk/articles/-A-534736