Determination of the remaining amounts of baquiloprim and trimethoprim residue in meat, liver and kidney by microbiological method
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. One of the priority areas of the state policy concerning public health nutritio is provision of foodstuff safety. Thus, features of their composition are taken into account, since, in addition to plastic material and biologically active substances, they can contain a lot of contaminants, including antimicrobials. The goal of the work was to conduct comparative analysis of the national and European microbiological screening methods for antibiotic residual detection in meat, liver and kidney testing and validation for qualitative microbiological “NAT-screening” method for baquiloprim and trimethoprim determination in meat liver and kidney. Material and methods. The sensitivity and specificity limits were established by the microbiological "NAT-screening" method for baquiloprim and trimethoprim residues determination. The study was carried out in 20 repetitions on simulated meat, liver and kidney samples which are free of antibiotics and also samples with introduced analyzed antibiotics solutions in concentrations of ½ MRL and MRL. Results of research and discussion. We found the clearly defined growth inhibition zone of pure test culture Bacillus pumilus CN 607, which ranged from 18.03±0.02 mm to 19.03±0.03 mm. The result of the microbiological screening method “NAT-screening” of baquiloprim and trimethoprim determination of meat revealed the following: for baquiloprim in a beef – 5 µg/kg, baquiloprim in pork – 20 µg/kg, trimethoprim in hors meat – 50 µg/kg, for a liver: 150 µg/kg of baquiloprim in a beef, 25 µg/kg of baquiloprim are in pork, for kidneys: baquiloprim in beef – 75 µg/kg, baquiloprim in pork – 25 µg/kg, which correspond to MDR of European law. Conclusions and prospects for further research. It was established that sensitivity, specificity and accuracy of the microbiological NAT-screening method for residual baquiloprim and trimethoprim is detection 100%.
Authors and Affiliations
Tetyana Garkavenko, Ilona Azyrkina, L. Shalimova, T. Kozitska
Keywords
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- EP ID EP388955
- DOI 10.31073/vet_biotech32(2)-09
- Views 142
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