DEVELOPMENT AND VALIDATION OF A SIMPLE AND RAPID RP- HPLC METHOD FOR THE DETERMINATION OF METRONIDAZOLE AND NORFLOXACIN IN COMBINED DOSAGE FORM
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 5
Abstract
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Metronidazole and Norfloxacin in pure and pharmaceutical dosage forms. Metronidazole and Norfloxacin were well separated using a Thermohypersil BDS C18, (5 μ, 150 x 4.6mm) and a mobile phase consisting of Phosphate buffer pH- 4.6: ACN (45:55) at the flow rate 1 ml/min and the detection was carried out at 254nm with UV detector. The Retention time for Metronidazole and Norfloxacin were found to be 2.440 & 4.510 respectively. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Metronidazole and Norfloxacin combination pharmaceutical dosage form.
Authors and Affiliations
SK Asma Parveen, Chandana Nalla
Keywords
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