Development and validation of a simultaneous HPLC method for Quantification of Hydrochlorothiazide and Propanolol Hydrochloride in Drug substance
Journal Title: Journal of Pharmacy Research - Year 2011, Vol 4, Issue 10
Abstract
A Reverse phase method has been developed for the quantitative estimation of Hct and Pnl-HCl in Drug Substance. The Quantification was carried out using RP stainless steel column ODS C18 250 x 4.6 x 5 µ L1 packing in Isocratic mode with mobile phase containing Acetonitrile: Water: Orthophosphoric acid (35: 64:1. Flow rate 1.0 ml/minute and the detection wavelength were set at 222 nm and the linearity was found to be in the range of 8-12 µg/ml for Hct and Pnl-HCl. The proposed method was found to be simple, precise, accurate, and reproducible for the estimation of Hct and Pnl-HCl
Authors and Affiliations
S a y y e d Hu s a i n a , Rashid R. Munjewar a , Mazahar Farooqui b
Keywords
Related Articles
- EP ID EP86669
- DOI -
- Views 156
- Downloads 0
Estimation of Bambuterol in tablet dosage forms by RP-HPLC
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Bambuterol in tablet dosage forms. An Inertsil ODS-3CV C18, 250x4.6 mm, coloumn with 5 µm particle size and the mobile p...
Antidiabetic and Antioxidant Effect of Ethanolic Root Extract of Boerhaavia diffusa in Streptatozocin-Induced Diabetic Rats
The ethanolic root extract of Boerhaavia diffusa have potent antidiabetic activity that reduces blood sugar level in Streptatozocin-Induced diabetic rats. The present study was carried out to investigate ntihypergl...
Emergence of ‘Super Bug’ and Use of Antibiotics: What We Learned, What We Have Yet to Learn?
Recent reports on emergence of a multidrug resistant ‘superbug’ have given the world a warning call against irrational use of antimicrobials. Few examples of most dangerous bugs include MRSA, Klebsiella Penumonia, vancom...
Development and validation of a simultaneous HPLC method for Quantification of Hydrochlorothiazide and Propanolol Hydrochloride in Drug substance
A Reverse phase method has been developed for the quantitative estimation of Hct and Pnl-HCl in Drug Substance. The Quantification was carried out using RP stainless steel column ODS C18 250 x 4.6 x 5 µ L1 packing in Is...
Various aspects of inflammation and the herbal drugs that are used for the treatment of inflammation: An overview
Inflammation is a body’s defence reaction to injury in order to eliminate or limit the spread of injurious agents followed by the removal of necrosed cells and tissues. Agents causing inflammation are physical agents (he...