Development and Validation of Simplified RP-HPLC Method for Quantification of Candesartan Cilexetil in Commercial Formulations
Journal Title: Der Pharma Chemica - Year 2024, Vol 16, Issue 1
Abstract
In order to produce antihypertensive effects, Candesartan Cilexetil (CC), a candesartan inactive prodrug, was quickly converted into active candesartan after absorption in the Gastrointestinal (GI) tract. This research study describes the development and validation of an accurate, precise, repeatable, easy and speedy Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) approach for measuring CC in a formulation using reverse-phase HPLC. In this study, liquid chromatography was performed on a Zorbax SB C-18 analytical column with dimensions of 2504.6 mm, 5 m, using a mobile phase consisting of Acetonitrile (ACN) and 0.1 percent orthophosphoric acid (pH 2.5) in a ratio of 35:65 vol/vol as the mobile phase. Results were presented as mean Standard Deviation (SD). This experiment used an injection volume of 20 microliters to measure the sample at 258 nano liters per minute while using a flow rate of 1.5 microliters per minute. 4.2 minutes was determined to be the retention time for both the reference and sample drugs. There was no evidence of nonlinearity in the calibration curve for CC when the concentration ranged from 50 ppm to 160 ppm and the Regression coefficient (R2 ) was determined to be 0.9996. When the percentage recovery of CC was achieved, it was in the range of 98.10 percent to 98.70 percent, indicating that the present approach was very accurate. Recovery trials using Percent Relative Standard Deviation (percent RSD) with intra and inter-day accuracy were found to be less than 2 percent, demonstrating that the established procedure is repeatable. The technique was verified in accordance with the International Conference on Harmonization (ICH) requirements and may be highly recommended for regular analysis of CC due to the fact that it is the most reliable and quick methodology currently available. Despite the fact that a great deal of research has been done on calculating CC in dosage forms, this study was determined to be under green chemistry conditions, as well as being quick and inexpensive. As a result, the statistical validation of the data revealed that the suggested approach may be used to estimate the CC in commercial formulations, which is encouraging.
Authors and Affiliations
Sachin Kothawade1 Rashmi Tambare Vishal Pande Rucha Pardeshi1 Sandesh Bole1 Pranaya Misar Balireddy Keesara Prachi Kanawade Kanchan Jadhav5 Vijaya Padwal Amit Lunkad and Manjusha Bhange
Keywords
Related Articles
- EP ID EP742481
- DOI 10.4172/0975-413X.16.1.197-203
- Views 29
- Downloads 0
Curcumin and Ascorbic Acid Therapeutic Benefits in a Female Wistar Rat with Renal Tissue UVB-Induced Hyperthyroidism: A Histological and Biochemical Study
Renal issues are a major problem for humanity. The renal system may sustain damage from the potentially dangerous radiation. Therefore, the purpose of this study is to evaluate how well curcumin and ascorbic acid protect...
Development and Validation of Simplified RP-HPLC Method for Quantification of Candesartan Cilexetil in Commercial Formulations
In order to produce antihypertensive effects, Candesartan Cilexetil (CC), a candesartan inactive prodrug, was quickly converted into active candesartan after absorption in the Gastrointestinal (GI) tract. This research s...
A Catalyst Free Simple and Efficient One Pot Synthesis of N-benzyloxazolidinone Derivatives
An efficient and catalyst free one pot synthesis of N-benzyloxazolidinone derivatives is developed by reaction of N-benzyl-β-amino alcohol derivatives and N, N-Carbonyldiimidazole (CDI) in DMSO. A range of novel N-benzyl...
β Sitosterol Quantification Using HPTLC in the Crude Seed Extracts for Potential Male Contraception Activity
β sitosterol is one of the main subcomponents of a group of plant sterols known as phytosterols that are very similar in composition to cholesterol. It is found to be present in many plant tissues like the leaves, roots,...
Recent Advancement of Phyto-Formulation in the Treatment of Neurodegenerative Diseases
Pharmacological molecular pathways during an epileptic episode and dementia were examined in the current review, which offers a helpful therapeutic approach for the treatment of neurological illnesses. The current resear...