DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC AREA UNDER CURVE METHOD FOR SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN COMBINED CAPSULE DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 2
Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical area under curve method for the simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in combined capsule dosage form. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s Rule”. Two analytical wavelength ranges selected were 251.0-261.0 nm and 276.0-286.0 nm for the estimation of Eperisone Hydrochloride and Diclofenac Sodium, respectively. The linearity of the proposed method was found in the range of 2-20 µg/ml for both Eperisone Hydrochloride and Diclofenac Sodium, respectively. The percentage mean recovery was found to be 101.54±0.25% for Eperisone Hydrochloride and 100.88±0.67% for Diclofenac Sodium. Also the method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less % Relative standard deviation value, indicating high grade of precision of the method. The method was successfully applied to pharmaceutical dosage forms because no interference from the capsule excipients was found.
Authors and Affiliations
Divyang Bhatt , Jawed Akhtar , Madhuri Baghel
Keywords
Related Articles
- EP ID EP120123
- DOI -
- Views 152
- Downloads 0
ACUTE TOXICITY, PHYTOCHEMISTRY AND ANTIBACTERIAL ACTIVITY OF AQUEOUS AND ETHANOLIC LEAF EXTRACTS OF CASSIA ALATA LINN
Cassia alata Linn is an important medicinal plant as well as an ornamental flowering plant that has diverse reported medicinal values. This study focused on the evaluation of the phytochemical components, antibacterial a...
EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF TWO ISOLATES FROM ASPILIA AFRICANA [PERS] C.D. ADAMS
Aspilia africana [PERS] C.D. Adams belong to the family of Asteraceae. It is a medicinal plant used by traditional herbal practitioners in Nigeria and some parts of West Africa for the treatment of various infections of...
FORMULATION AND EVALUATION OF LOSARTAN POTASSIUM AND HYDROCHLORTHIAZIDE CONVENTIONAL RELEASE TABLETS
The losartan potassium and hydrochlorthiazide tablets are prepared by using low substituted HPC as disintegrant. The addition of diuretics to angiotensin II receptor blockers will potentiate the action of angiotensin rec...
ASYSTASIA GANGETICA: REVIEW ON MULTIPOTENTIAL APPLICATION
Asystasia gangetica (L).T. also known as “Chinese violet” is a rapidly growing straggling herb mainly distributed in India. In traditional system of medicine, the plant is used for various ailments and diseases. The pres...
FACTORS AFFECTING PHARMACOKINETIC DISPOSITION OF DRUGS
Absorption of drugs from the gastrointestinal tract is a complex process the variability of which is influenced by many physicochemical and physiologic factors. The two most important physicochemical factors that affect...