DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC AREA UNDER CURVE METHOD FOR SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN COMBINED CAPSULE DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 2
Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical area under curve method for the simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in combined capsule dosage form. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s Rule”. Two analytical wavelength ranges selected were 251.0-261.0 nm and 276.0-286.0 nm for the estimation of Eperisone Hydrochloride and Diclofenac Sodium, respectively. The linearity of the proposed method was found in the range of 2-20 µg/ml for both Eperisone Hydrochloride and Diclofenac Sodium, respectively. The percentage mean recovery was found to be 101.54±0.25% for Eperisone Hydrochloride and 100.88±0.67% for Diclofenac Sodium. Also the method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less % Relative standard deviation value, indicating high grade of precision of the method. The method was successfully applied to pharmaceutical dosage forms because no interference from the capsule excipients was found.
Authors and Affiliations
Divyang Bhatt , Jawed Akhtar , Madhuri Baghel
Keywords
Related Articles
- EP ID EP120123
- DOI -
- Views 160
- Downloads 0
ANTI-INFLAMMATORY AND ANTIPYRETIC EFFECTS OF CARPOLOBIA LUTEA LEAF EXTRACT IN RODENTS
The polar and non-polar fractions from the leaf of Carpolobia lutea G. Don (Family Polygalaceae) were assessed for anti-inflammatory and antipyretic effects. Anti-inflammatory activities were evaluated using acute anti-i...
HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF FICUS MOLLIS ON PARACETAMOL INDUCED LIVER DAMAGE IN ALBINO RATS
The present study was conducted to evaluate the hepatoprotective activity of alcoholic extract of Ficus mollis stem bark against paracetamol induced liver damage in Albino rats. The alcoholic extract of stem bark of Ficu...
LINEAR ALKYL BENZENE SULPHONATE, A DETERGENT INDUCED TOXICITY ON THE GILL OF PUNTIUS TICTO FISH
Detergents are common household products used for cleaning of domestic materials. The ‘after wash’ of the detergents are either drained into the aquatic environment such as ponds, lakes, rivers, streams, etc. or they fin...
SUSTAIN OPHTHALMIC DELIVERY OF LEVOFLOXACIN FROM A pH -TRIGGERED IN SITU GELLING SYSTEM
Most eye diseases are treated with topical application of eye drops. The poor bioavailability and therapeutic response exhibited by these conventional eye drops due to rapid precorneal elimination of the drug may be over...
FORMULATION AND EVALUATION OF MOUTH DISSOLVING TABLETS OF METFORMIN HYDROCHLORIDE
Metformin HCl is a biguanide antihyperglycemic agent used for treating non-insulin-dependent diabetes mellitus (NIDDM). It improves glycemic control by decreasing hepatic glucose production, decreasing glucose absorption...