DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC AREA UNDER CURVE METHOD FOR SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN COMBINED CAPSULE DOSAGE FORM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 2
Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical area under curve method for the simultaneous estimation of Eperisone Hydrochloride and Diclofenac Sodium in combined capsule dosage form. The proposed area under curve method involves the measurement of area at selected analytical wavelength ranges and performing the analysis using “Cramer’s Rule”. Two analytical wavelength ranges selected were 251.0-261.0 nm and 276.0-286.0 nm for the estimation of Eperisone Hydrochloride and Diclofenac Sodium, respectively. The linearity of the proposed method was found in the range of 2-20 µg/ml for both Eperisone Hydrochloride and Diclofenac Sodium, respectively. The percentage mean recovery was found to be 101.54±0.25% for Eperisone Hydrochloride and 100.88±0.67% for Diclofenac Sodium. Also the method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less % Relative standard deviation value, indicating high grade of precision of the method. The method was successfully applied to pharmaceutical dosage forms because no interference from the capsule excipients was found.
Authors and Affiliations
Divyang Bhatt , Jawed Akhtar , Madhuri Baghel
Keywords
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