Effect of Chitosan on Physical status of Mammals and in vivo Antimicrobial Effect on Pathogenic Bacteria
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 1
Abstract
The shells as raw materials were extracted from shrimp (Metapenous Monoceros) to extract chitosan in this experiment. Demineralizations process was carried out by 4% HCl at room temperature in the ratio of 1:14(w/v). The deproteinization process was initiated by 5% NaOH at 90°C for 24 hours with a solvent to solid ratio of 12:1(v/w). Removal of acetyl groups from the chitin was achieved by using 70% NaOH solution with a solid to solvent ratio of 1:14 (w/v) at room temperature for 72 hours. Extracted chitosan was soluble in 1% acetic acid. Rabbit were treated with extracted chitosan to determine in vivo effects. 2.74g of chitosan was up taken by each rabbits per day. 80gm of standard food was given to each rabbit during experiments. Physical status was observed and stool samples were collected to observe antimicrobial before chitosan treatment and after chitosan treatment. Presence of pathogenic bacteria was detected by streaking loop-full sample on three specific media. Coliform detection was initiated by MacConkey Agar. Salmonella-Shigella detection was initiated by S-S Agar. Pseudomonas detection was initiated by Cetrimide Agar. Before chitosan treatment 2300g body weight was found, stool status was rigid and physical movement was normal. After chitosan treatment a continuous bacterial growth in the 7th, 10th, 15th day but none of Coliform and Salmonella-Shigella growth occurred that indicates a ratio of growth of normal microbial flora. The physical status of the rabbit was changed during chitosan treatment. Continuous reduction in weight and looseness of stool was observed, but physical movement was normal.
Authors and Affiliations
Sazin Islam, Sharmin Akter, Md. Mohbub Alam1, Md. Mizanur Rahman3 ,, Md. Golam Sorwar, Suvamoy Datta4
Keywords
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- EP ID EP383299
- DOI 10.9790/3008-11125055 .
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