Factors Affection Degradation of Poly-3-Hydroxybutyrate (Bioplastic) By A Filamentous Bacterium of The Genus Streptomyces
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 6
Abstract
Actinomycetes are filamentous Gram-positive bacteria mostly found in soil and are known as a best producer of antibiotics and useful enzymes. They have important role in the breaking dawn of organic materials and polymers. About 40 actinomycete isolates were obtained from saline soil collected from Jeddah on starch nitrate agar using serial dilution method. All isolates were screened for poly-3-hydroxybutyrate (PHB) degradation on agar and broth media containing PHB as carbon source. The isolate GHM20 was the most active isolate in PHB degradation and using different characteristic, it was identified as species belong to genus Streptomyces. Identification was confirmed and the isolate was identified as Streptomyces sp. GHM20. The best degradation was recorded at pH 6.5, 25ᵒC after 3 days of incubation in minimal medium containing 2 g/l glucose and 0.2 g/l yeast extract as additional carbon and nitrogen sources, respectively. The used medium contained 1.5% PHB enhanced degradation of PHB and depolymerase production. In conclusion, the species belonging to the genus Streptomyces showed excellent activities in PHB degradation and improving growth conditions enhanced the degradation process and depolymerase production.
Authors and Affiliations
Magda M. Aly, Ghofran Nezar Redwan, Lubna Nawar
Keywords
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- EP ID EP392472
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