Effect of Different Vector and Host Combinations on Recombinant Protein Expression
Journal Title: 河南科技大学学报（自然科学版） - Year 2016, Vol 37, Issue 2
Abstract
The effect of vector-host combination on the expression level of recombination proteins in Pichia pastoris system was studied. Five recombination proteins including onconase( ONC),IL-1α,IL-1β,IL-1 Ra and HSA-( Gly4Ser1)_3-ONC( HSA-ONC) fusion protein were inserted into p PIC9,p PIC9 K and p PICZα-A,then the recombination vectors were respectively transformed into three P. pastoris strains( X-33,GS115 and SMD1168). Seven kinds of vector-host combinations were obtained after being screened,and these five recombination proteins were induced and quantitified in the flask scale. The results show that the expression level of five fusion proteins are the highest in p PICZα-A / X-33,but the lowest in p PIC9 / SMD1168. The p PICZα-A / X-33 system is better than the other six combinations based on the yield of recombination proteins.
Authors and Affiliations
Ganggang YANG, Ze WANG, Haijing BAI, Yajuan YANG, Cunshuan XU
Keywords
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